| Staurosporine对CMK细胞增殖和分化的影响 |
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| 引用本文： | 赵美蓉,许一多.Staurosporine对CMK细胞增殖和分化的影响[J].中国免疫学杂志,2003,19(5):313-314. |
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| 作者姓名： | 赵美蓉 许一多 |
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| 作者单位： | 吉林大学第三医院肿瘤内科,长春,130021 |
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| 摘 要： | 目的：探讨Staurosporine(ST)对原始巨核细胞白血病细胞系(CMK)的增殖分化作用。方法：采用MTT分析方法及流式细胞仪测定细胞周期分布。结果：ST呈浓度依赖性地抑制CMK细胞的增殖，IC50为50nmol/L；ST可将CMK细胞阻抑在G1期，从而减少S和G2M期的细胞；以巨核细胞分化特异性膜表面抗原CD41为指标，80nmol/L的ST可使CMK细胞CD41抗原表达率明显增加。结论：ST可抑制CMK细胞的增殖，诱导其分化，ST作为一种新的抗肿瘤因子具有与其他抗肿瘤药物不同的作用机制。
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| 关 键 词： | Staurosporine CMK细胞增殖 分化 影响 |
| 文章编号： | 1000-484X(2003)05-0313-02 |
The expansion and differentiation effects of Staurosporine on CMK cell |
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| ZHAO Mei-Rong,XU Yi-Duo.The expansion and differentiation effects of Staurosporine on CMK cell[J].Chinese Journal of Immunology,2003,19(5):313-314. |
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| Authors: | ZHAO Mei-Rong XU Yi-Duo |
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| Institution: | ZHAO Mei-Rong,XU Yi-Duo.Department of Oncology,Third Affiliated Hospital of Jilin University,Changchun 130021,China[ |
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| Abstract: | Objective:To determine the expansion and differentiation effect of Staurosporine on human megalaryoblastic cell line(CMK).Methods:With MTT and colony assaies.Results:Staurosporine could densitily inhibited the expansion of CMK cell,IC 50 was 50 nmol/L,Staurosporine could inhibite CMK cell at G 1 phase,then decreased the cells of S and G 2M phases,ST in 80 nmol/L improve the expression of CD41 antigen in CMK cell,induce its differentiation.Conclusion:ST as a new kind of anti-tumor facor has different mechanism with other anti-tumor drugs. |
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| Keywords: | Staurosporine(ST) CMK cell Expansion and differentiation |
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