三七总皂苷预处理对炎性内脏痛大鼠脊髓背角谷氨酰胺合成酶表达的影响

目的探讨三七总皂苷(PNS)预处理调节脊髓背角谷氨酰胺合成酶(GS)表达,进而影响大鼠炎性内脏痛的病理生理机制。方法将27只SD大鼠随机分为正常对照组、PNS预处理组、溶媒对照组,给予相应处理15d后腹腔注射1%乙酸(10ml/kg)制作模型,每隔15min记录内脏痛指数;取小肠组织进行HE染色,光学显微镜下观察炎症反应;应用免疫组织化学技术检测胸段脊髓背角GS表达情况。结果溶媒对照组内脏痛指数明显高于PNS预处理组;腹腔注射乙酸后小肠组织都有明显的炎症反应,但两实验组黏膜下层厚度无明显差异;两实验组脊髓背角GS表达较正常组明显降低,但PNS预处理组脊髓背角GS表达明显高于溶媒对照组。结论 PNS预处理能上调脊髓后角GS的表达,从而缓解乙酸所致炎性内脏痛。

Panax notoginseng saponins pretreatment affects the inflammatory visceral pain via upregulation of the glutamine synthetase in the rat spinal dorsal horn

Objective To explore the effect of panax notoginseng saponins (PNS) pretreatment on the expression of glutamine synthetase (GS) in the inflammatory visceral pain in the rat spinal dorsal horn. Methods Twenty-seven SD rats were randomly divided into normal group, PNS pretreatment group, and vehicle control group. Fifteen days after pretreatment, the model of inflammatory visceral pain was made by intraperitoneal injection of 1% acetic. The visceral pain index of experimental rat was record every 15 minutes. The small intestine tissue was stained with HE. The thoracic spinal cord slices were immunostained with anti-glutamine-synthetase antibody, then observed and compared under an optical microscope. Results The score result of the vehicle control group was significantly higher than that of the PNS pretreatment group. Compared with the normal group, the small intestine tissues of the two experimental groups showed significantly inflammatory response with thickness submucosa, but there was no significantly difference among experimental comparison groups. Compared with the normal group, the GS expression of the two experimental groups was reduced apparently, but the group of PNS pretreatment was significantly higher than the vehicle control group. Conclusion PNS pretreatment may affect the acid-induced inflammatory visceral pain via promoting the experience of GS in the spinal dorsal horn.