| 转染BMP-2基因的兔BMSCs种植PLA/PCL支架体外构建组织工程骨 |
| |
| 引用本文： | 卜丽莎,李建军,高申,杨绍娟,张文岚,崔亚南,刘建国,徐莘香.转染BMP-2基因的兔BMSCs种植PLA/PCL支架体外构建组织工程骨[J].中国矫形外科杂志,2004,12(9):677-679. |
| |
| 作者姓名： | 卜丽莎 李建军 高申 杨绍娟 张文岚 崔亚南 刘建国 徐莘香 |
| |
| 作者单位： | 1. 吉林大学中日联谊医院中心研究室,长春,130033
2. 吉林大学第一医院骨科 |
| |
| 基金项目： | 国家自然科学基金，吉林省科技厅科研项目 |
| |
| 摘 要： | 目的:用腺病毒载体将人骨形态发生蛋白-2(BMP-2)基因导入兔骨髓基质干细胞(BMSCs),种植PLA/PCL(聚乳酸/聚己内酯)支架体外构建组织工程骨.方法:蛋白印迹法检测转染后细胞BMP-2的表达,流式细胞仪和ALP活性检测分析基因转染对细胞增殖分化的影响.然后将转染后细胞接种到PLA/PCL支架上,扫描电镜观察细胞贴附、生长状况.结果:转染后,BMSCs表达BMP-2,S期细胞比例和ALP活性明显增高.扫描电镜见转染细胞分布均匀,伸展良好.结论:BMP-2基因转染BMSCs,可促进细胞增殖分化.转染后细胞在PLA/PCL支架上生长良好,BMP-2基因治疗的组织工程骨构建成功.
|
| 关 键 词： | 腺病毒 骨形态发生蛋白 骨髓基质干细胞 组织工程骨 |
| 文章编号： | 1005-8478(2004)09-0677-03 |
| 修稿时间： | 2003年9月9日 |
Bone marrow stromal cells carrying human bone morphogenetic protein-2 gene seeded into PLA/PCL scaffold to construct tissue engineering bone in vitro |
| |
| BU Li sha,LI Jian jun,GAO Shen,et al Central Research Lab,China Japan Union Hospital,Jilin University,Changchun.Bone marrow stromal cells carrying human bone morphogenetic protein-2 gene seeded into PLA/PCL scaffold to construct tissue engineering bone in vitro[J].The Orthopedic Journal of China,2004,12(9):677-679. |
| |
| Authors: | BU Li sha LI Jian jun GAO Shen Central Research Lab China Japan Union Hospital Jilin University Changchun |
| |
| Institution: | BU Li sha,LI Jian jun,GAO Shen,et al Central Research Lab,China Japan Union Hospital,Jilin University,Changchun 130033 |
| |
| Abstract: | Objective:Rabbit bone marrow stromal cells (BMSCs) infected by a recombinant adenoviral vector carrying the human BMP 2 gene (Ad BMP 2) were seeded into PLA/PCL scaffolds to construct tissue engineering bone in vitro Method:The expression of hBMP 2 in these cells after infection was determined by Western blot analysis The changes of cellular proliferation and differentiation were observed by flow cytometer and ALP activity analysis BMP 2 transduced cells were then seeded into PLA/PCL scaffolds The attachment and growth of the cells on the scaffold were examined using SEM Result:The expression of hBMP 2 was confirmed and ALP activity obviously increased in the cells after infection SEM examination revealed extensive cellular attachment and growth on the PLA/PCL block Conclusion:Ad BMP 2 could infect BMSCs with high efficiency and promote its diferentiation The cells after infection grow well on PLA/PCL scaffold Tissue enginerring bone used to regional gene therapy is constructed successfully |
| |
| Keywords: | Adenoviral vector Bone morphogenetic protein(BMP) Bone marrow stromal cells(BMSCs) Tissue engineering bone |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
