1,3-Dichloropropene: Two-Generation Inhalation Reproduction Study in Fischer 344 Rats

1,3-Dichloropropene: Two-Generation Inhalation ReproductionStudy in Fischer 344 Rats. BRESLIN, W. J., KIRK, H. D., STREETER,C. M., QUAST, J. F.,AND SZABO, J. R. (1989). Fundam. Appl Toxicol.12, 129–143. This study evaluated the effects of inhaledtechnical-grade 1,3-dichloropropene (DCPT) on reproduction andneonatal growth and survival. Groups of 30 male and 30 femaleFischer 344 rats, approximately 6 weeks of age, were exposedvia inhalation to 0, 10, 30 or 90 ppm DCPT for 6 hr/day, 5 days/week,for two generations. The parental f₀ and f₁ generations wereeach bred twice. Reproductive and neonatal parameters evaluatedincluded indices of fertility and pup survival, gestation length,litter size, pup body weight, and pup sex ratio. Gross and histologicexaminations were conducted on all f₀ and f₁ adults. In addition,randomly selected f_1b and f_2b weanlings were given gross examinations.Parental effects were limited to rats exposed to 90 ppm DCPTand included decreased body weights and histopathologic effectson the nasal mucosa of adult male and female rats. The histopathologiceffects consisted of slight, focal hyperplasia of the respiratoryepitheium and/or focal degenerative changes in the olfactoryepithelium. No adverse effects on reproductive parameters orneonatal growth or survival were observed in the f_1a, f_1b, f_2a,or f_2b litters even at an exposure concentration which producedeffects in adult animals. Based on these results, it is concludedthat inhalation exposure of rats up to 90 ppm DCPT for two successivegenerations did not adversely affect the reproductive and neonatalparameters evaluated.