Apoptotic and antiproliferative properties of 3β‐hydroxy‐Δ5‐steroidal congeners from a partially purified column fraction of Dendronephthya gigantea against HL‐60 and MCF‐7 cancer cells |
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Authors: | I P Shanura Fernando K K Asanka Sanjeewa Hyun‐Soo Kim Lei Wang Won Woo Lee You‐Jin Jeon |
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Institution: | Department of Marine Life Science, Jeju National University, Jeju, Republic of Korea |
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Abstract: | Organisms belonging to the genus Dendronephthya are among a group of marine invertebrates that produce a variety of terpenoids with biofunctional properties. Many of these terpenoids have been proven effective as anticancer drugs. Here, we report the antiproliferative effect of 3β‐hydroxy‐Δ5‐steroidal congeners against the proliferation of HL‐60 human leukemia cells and MCF‐7 human breast cancer cells. The sterol‐rich fraction (DGEHF2‐1) inhibited the growth of HL‐60 and MCF‐7 cells with IC50 values of 13.59 ± 1.40 and 29.41 ± 0.87 μg ml–1 respectively. Treatment with DGEHF2‐1 caused a dose‐dependent increase in apoptotic body formation, DNA damage and the sub‐G1 apoptotic cell population. Moreover, DGEHF2‐1 downregulated the expression of Bcl‐xL while upregulating Bax, caspase‐9, and PARP cleavage in both HL‐60 and MCF‐7 cells. The steroid fraction was found to act via the mitochondria‐mediated apoptosis pathway. Identification of the sterols was performed via gas chromatography–tandem mass spectrometry analysis. Studying the mechanism of the anticancer effect caused by these sterol derivatives could lead to the identification of other natural products with anticancer properties. |
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Keywords: | Antiproliferative apoptosis Dendronephthya gigantea HL‐60 Jeju MCF‐7 soft coral |
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