骨髓单个核细胞与骨髓间充质干细胞促进大鼠皮瓣存活率的比较 |
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引用本文: | 许鹏,唐郑雅,陆阳,周广东,刘伟,曹谊林,张文杰.骨髓单个核细胞与骨髓间充质干细胞促进大鼠皮瓣存活率的比较[J].组织工程与重建外科,2015,11(2):90-94. |
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作者姓名: | 许鹏 唐郑雅 陆阳 周广东 刘伟 曹谊林 张文杰 |
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作者单位: | 上海交通大学医学院附属第九人民医院整复外科,上海市组织工程研究重点实验室;组织工程国家工程中心 |
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基金项目: | 国家重点基础研究发展计划(“973”项目)(2011CB964704);国家自然科学基金(81271714,31170944) |
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摘 要: | 目的比较来源于等量骨髓的单个核细胞与经扩增的间充质干细胞,促进大鼠随意皮瓣成活率的效果。方法取等质等量的大鼠骨髓,一半直接离心获得骨髓单个核细胞,一半经体外培养获得骨髓间充质干细胞,注射相等数量(n=6)的大鼠随意皮瓣。术后测量并计算皮瓣成活面积,取材,行组织学检测,计数CD31阳性血管数量。结果未经培养的骨髓单个核细胞组皮瓣的平均存活率为(71.6±8.4)%,培养的骨髓间充质干细胞组平均存活率为(66.2±3.1)%,这两组存活率均显著高于注射平衡液的对照组(55.9±3.4)%;注射细胞组之间平均存活率没有统计学差异。组织学血管密度计数显示,骨髓单个核细胞组和骨髓间充质干细胞组的微血管数量分别是(58.2±6.8)和(42.7±5.1),都显著高于PBS对照组(22.8±3.1),而骨髓单个核细胞组也显著高于骨髓间充质干细胞组。结论与不经培养的骨髓单个核细胞相比,通过体外扩增的骨髓间充质细胞,未能显著提高大鼠随意皮瓣的成活率。
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关 键 词: | 随意皮瓣 细胞治疗 骨髓单个核细胞 骨髓间充质干细胞 |
Comparison between Bone Marrow-derived Mononuclear Cells and Mesenchymal Stem Cells in Promoting Survival Rates of Random-pattern Skin Flap in Rats |
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Authors: | XU Peng;TANG Zhengya;LU Yang;ZHOU Guangdong;LIU Wei;CAO Yilin;ZHANG Wenjie |
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Institution: | Department of Plastic and Reconstructive Surgery, Shanghai Ninth People’s Hospital, Shanghai Jiaotong University School of Medicine, Shanghai Key Laboratory of Tissue Engineering;National Tissue Engineering Center of China; |
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Abstract: | Objective To compare the non-cultured bone marrow mononuclear cells (BM-MNCs) and cultured bone marrow mesenchymal stem cells (BMSCs) in the promotion of random-pattern skin flap survival of rats. Methods BM-MNCs and BMSCs derived from two identical rat bone marrow aspirates were injected in a random-pattern skin flap model of rat (n=6). The survival area was determined by its appearance, color and texture. Capillary density of flaps was measured by histology. Results The flap survival rates were (71.6±8.4)%in the BM-MNC-treated group and (66.2±3.1)%in the BMSC-treated group, both of which were significantly higher than the control group (55.9 ±3.4)%. However, no significant difference was observed between the cell transplanted groups. According to vessel density assay, capillary density in the BM-MNC-treated group (58.2±6.8) was higher than in the BMSC-treated group (42.7±5.1), both of which were significantly higher than the control group (22.8±3.1). Conclusion Pre-culture of BMSCs does not bring therapeutic benefits. |
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Keywords: | Random-pattern skin flap Cell therapy Bone marrow mononuclear cells Bone marrow mesenchymal stem cells |
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