星型M-PLGA纳米药物制剂用于宫颈癌治疗的研究

目的 本研究合成了一种星型的甘露醇引发的聚（乳酸-羟基乙酸）共聚物（M-PLGA）,旨在提供一种新型的纳米制剂用于宫颈癌的治疗.方法 这种新型的共聚物通过开环聚合合成,利用核磁共振仪进行表征.采用改进的纳米沉淀法制备载多烯紫杉醇M-PLGA纳米粒并在扫描电镜下观察纳米粒的形态.结果 M-PLGA纳米粒粒径分布较窄,在人宫颈癌Hela细胞中的摄取水平要高于PLGA纳米粒.载多烯紫杉醇的M-PLGA纳米粒对Hela细胞的毒性显著高于商用的泰素帝和载多烯紫杉醇的PLGA纳米粒,证明星型M-PLGA聚合物作为纳米药物载体优于线型PLGA聚合物;同时,星型M-PLGA的载药量也明显高于线型聚合物.结论 星型M-PLGA共聚物可作为一种极具潜力的用于宫颈癌治疗的纳米载体材料.

Docetaxel-loaded nanoformulation based on star-shaped M-PLGA for cervical cancer therapy

Objective Mannitol-poly（D,L-lactide-co-glycolide） （M-PLGA）,a star-shaped biodegradable polymer,was synthesized with the intent in this research,to provide novel nanoformulation for cervical cancer chemotherapy.Methods The novel star-shaped copolymer was prepared by ring-opening polymerization,and characterized by NMR.The docetaxel-loaded M-PLGA nanoparticles,prepared by modified nano-precipitation method,were observed to be near-spherical shape with narrow size distribution.Results The CLSM results showed the uptake level of M-PLGA NPs was higher than PLGA NPs in Hela cells.A significantly higher level of cytotoxicity was achieved by docetaxel-loaded M-PLGA NPs than that of commercial Taxotere and docetaxel-loaded PLGA NPs,indicating that the star-shaped biodegradable polymer M-PLGA could be superior to the linear polymer PLGA as a drug carrier.The study on drug loading and encapsulation efficiency also proved that star-shaped M-PLGA could carry higher level of drug than linear polymer,therefore could be more efficient for cancer treatment.Conclusions In conclusion,the star-shaped M-PLGA copolymer may be used as a potential and promising drug-loaded biomaterial applied in developing novel nanoformulations for cervical cancer therapy.