血管内皮生长因子及骨形态发生蛋白2对成骨细胞生长的促进作用

目的研究血管内皮生长因子（vascular endothelial growth factor,VEGF）以及骨形态发生蛋白2（bone morphogendtic protein 2,BMP-2）对成骨细胞增殖的调节作用。方法提取人骨髓间充质干细胞（mesenchymal stem cells,MSCs）并使其分化为成骨细胞,分别添加不同浓度的VEGF和BMP-2进行药物干预,用四甲基偶氮唑盐（MTT）法研究成骨细胞的增殖率。结果两组干预组的吸光度（A）值与对照组的A值相比较,差异有统计学意义（P〈0.05）,对成骨细胞增殖的调节作用均随剂量和时间变化而改变,两组调节作用的最佳时间均为72 h左右。两组最佳作用浓度分别为：BMP-2为1.8×10-9 mol/L,对成骨细胞的增殖率达179.59%;VEGF为1×10^-8 mol/L,其增殖率达189.80%。结论 VEGF对成骨细胞增殖的促进作用不亚于BMP-2。

Promoting effect of VEGF and BMP-2 on growth of osteoblasts

Objective To study the regulatory effects of vascular endothelial growth factor（VEGF）and bone morphogendtic protein 2（BMP-2）on proliferation of osteoblasts.Methods The human bone marrow mesenchymal stem cells（MSCs）were isolated and differentiated into osteoblasts,and different concentrations of VEGF and BMP-2 were added for drug intervention.MTT assay was used to examine the proliferation of osteoblasts.Results There was significant difference in aborbance values between intervention group and control group（P0.05）.The regulatory effects of VEGF and BMP-2 on proliferation of osteoblasts were time-and concentration-dependent.The optimal time was about 72 h in both two groups.BMP-2 1.8×10^-9 mol/L obtained proliferation rate of 179.59%,and VEGF1×10-8 mol/L obtained proliferation rate of 189.80%.ConclusionThe promoting effect of VEGF on proliferation of osteoblasts is similar to that of BMP-2.