雷公藤抑制免疫排斥反应的实验研究

目的：通过雷公藤孵化皮片，在同种小鼠间进行移植，探讨雷公藤对免疫排斥反应的抑制作用。方法：采用１５％雷公藤煎剂和４０μｇ／ｍｌ、１００μｇ／ｍｌ、５００μｇ／ｍｌ雷公藤总甙对供鼠皮片浸泡孵化，在昆明种小鼠和ＤＢＡ褐色小鼠间进行皮片移植，观察雷公藤对同种皮片移植免疫排斥的抑制作用。结果：雷公藤煎剂孵化皮片能显著延长移植物的存活时间，两种小鼠皮片移植后存活时间分别为（２２３１±３６０）天、（２１５０±３４０）天，与对照组（１３２０±２２５）天、（１１８１±２７５）天相比有显著意义（Ｐ＜００１）；４０μｇ／ｍｌ雷公藤总甙孵化后皮片存活时间为（１７５４±１４４）天，１００μｇ／ｍｌ为（１９８４±２６３）天，５００μｇ／ｍｌ为（２０９１±３８０）天；雷公藤总甙孵化加腹腔注射，每天４０μｇ／２０ｇ小鼠为（２１３５±２０８）天，１００μｇ为（２２３４±２７５）天，５００μｇ为（１９８２±１１４）天，与对照组（１２８３±０８０）天、（１１９６±１７５）天相比均有显著差异（Ｐ＜００１）；雷公藤总甙单纯孵化各组与孵化加腹腔注射相比，除４０μｇ／ｍｌ组（Ｐ＜００５）外，存活时?

Experimental Research of Thunder Godvine to Suppress Immune Rejection

Objective:To study the suppression of thunder godvine (tripterygiun wilfordii) to immunological rejective reaction during the transplant of isotype mice with hatched skin graft of thunder godvine. Methods:15% thunder godvine boiled solutions, and 40 μg/ml, 100 μg/ml, 500 μg/ml of thunder godvine glucoside were used to soak and hatch the mice skin graft, and then transplant the skin graft between Kunming-type mice and DBA brownish mice,so as to observe the suppression of thunder godvine to immunological rejection of isotype skin graft.Results: It is obvious that the hatched skin grafts of thunder godvine boiled solutions could prolong the surviving time of the hemotransplant, the surviving time of two kinds of mice was (22.31±3.60) days, (21.50±3.40) days respectively, there was significant difference ( P <0.01), as compared with the control group(13.20±2.25) days, (11.82±2.75) days. The skin graft survivaltime was (17.54±1.44) days.with 40 μg/ml thunder godvine glucoside eclosion; (19.84±2.63) days with 100 μg/ml eclosion; (20.91±3.80) days with 500 μg/ml eclosion. Every day 40 μg/20g of thunder godvine glucoside was used to hatch and inject the mice for (21.35±2.08) days; 100 μg for (22.34±2.75) days and 500 μg for (19.82±1.14) days.It had significant difference( P <0.01) as comparision of pure eclosion of each group of thunder godvine glucoside with the eclosion together with cavity injection except 40 μg/ml group ( P <0.05)], the survival period had no remarkable differences ( P <0.05). Conclusion:Thunder godvine colud obviously prolong the skin gragt survival period and obtain satisfactory results by was of simple eclosion。