血小板活化因子致心肌缺血与离子转运、脂质过氧化的关系

探讨血小板活化因子（ＰＡＦ）所致心肌缺血与钙离子积聚以及脂质过氧化的关系。方法大鼠经腹腔注入ＰＡＦ，２０ｍｉｎ后处死，测定其红细胞膜钠、钙泵活性、ＣＡＴ酶活性以及ＬＰＯ含量；同时测定银杏内酯Ｂ拮抗ＰＡＦ后对膜钠、钙泵、ＣＡＴ酶活性以及ＬＰＯ含量的影响。结果大鼠经注入ＰＡＦ后心肌形成缺血，红细胞膜钠泵活性降低；钙泵、ＣＡＴ酶活性以及ＬＰＯ含量均升高。经银杏内酯Ｂ拮抗ＰＡＦ后，除钠泵未有改善外，钙泵、ＣＡＴ酶活性以及ＬＰＯ含量均恢复或接近正常水平。结论ＰＡＦ所致心肌缺血可能与自由基产生以及钙受体有关。

Relationship Between Platelet Activating Factor Induced Myocardial Ischemia,Ion Transport and Lipid Peroxidation

Purpose To investigate the relationship between PAF induced myocardial ischemia,calcium ion aggregate and lipid peroxidation. Methods Rats were injected with PAF via the abdominal cavity and were executed after 20 min.The activities of erythrocyte membrane sodium pump,calcium pump and CAT were determined with the aid of ammonium molybdate method and dynamic method respectively,and the content of LPO was assayed through the use of spectrophotometry of thiobarbituric acid. Results We found that the myocardial ischemia of rat was induced by injection of PAF,the activities of erythrocyte membrane sodium pump was decreased.The activities of calcium pump and CAT increased and the content of LPO increased.The results from the use of the ginkgolide B to antagonise the PAF showed that the activities of calcium pump and CAT,and the content of LPO returned to normal. Conclusions The PAF induced myocardial ischemia may be related to the production of free radical and calcium receptor.