梅毒患者白介素4和Toll样受体2基因单核苷酸多态性研究

目的 探讨IL-4基因-590C/T位点、Toll样受体2(TLR2)基因Arg753Gln位点单核苷酸多态性与梅毒遗传易感性之间是否存在关联.方法 分别采用实时荧光定量PCR技术和DNA测序法检测56例早期显性梅毒患者、38例早期潜伏梅毒患者和102例正常人对照组IL-4基因-590C/T位点、TLR2基因Arg753Gln位点单核苷酸多态性.结果 早期梅毒组IL-4-590位点C、T等位基因的分布频率分别为0.229、0.771,对照组IL-4-590位点C、T等位基因的分布频率分别为0.216、0.784,两组相比差异无统计学意义(P＞0.05);显性梅毒组IL-4-590位点C、T等位基因的分布频率分别为0.232、0.768,潜伏梅毒组IL-4-590位点C、T等位基因的分布频率分别为0.224、0.776,两组相比差异无统计学意义(P＞0.05).各组TLR2Arg753Gln位点基因型均为GG型,GA、AA型缺如.早期梅毒组TLR2 Arg753Gln位点G、A等位基因的分布频率与对照组TLR2 Arg753Gln位点G、A等位基因的分布频率相比差异无统计学意义;显性梅毒组TLR2 Arg753Gln位点G、A等位基因的分布频率与潜伏梅毒组TLR2 Arg753Gln位点G、A等位基因的分布频率相比差异无统计学意义.结论 IL-4基因-590C/T位点、TLR2基因Arg753Gln位点单核苷酸多态性与梅毒遗传易感性无相关性.

Single nucleotide polymorphism of interleukin-4 (IL-4) gene and Toll-like receptor 2 (TLR2) gene in patients with syphilis

Objective To assess the relationship of IL-4 -590C/T polymorphism and TLR2 Arg753Gln polymorphism with genetic susceptibility to syphilis. Methods The study included 56 patients with early symptomatic syphilis, 38 patients with early latent syphilis and 102 normal human controls. Real-time quantitative fluorescent PCR was used to detect the IL-4 -590C/T polymorphism, and DNA sequencing to analyze the TLR2 Arg753Gln polymorphism, in these subjects. Results The frequency of C and T alleles at 590 position of IL-4 gene was respectively 0.229 and 0.771 in patients with early syphilis, 0.216 and 0.784 in the normal controls, 0.232 and 0.768 in patients with early symptomatic syphilis, 0.224 and 0.776 in patients with early latent syphilis. GG genotype was observed at position 753 in TLR2 gene in all the subjects. No significant difference was observed for the frequency of C or T alleles at position 590 in IL-4 gene or the frequency of A or G alleles at position 753 in TLR2 gene between patients with early syphilis and normal controls or between patients with early latent syphilis and those with early symptomatic syphilis (all P ＞ 0.05).Conclusion There seems no evident association between the susceptibility to syphilis and IL-4 -590C/T polymorphism or TLR2 Arg753Gln polymorphism.