| rAAV/BA46转染树突状细胞诱导特异细胞免疫 |
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| 引用本文： | 尤长宣,苏瑾,廖旺军,张军一,Liu Yong,Paul L Hermonat,罗荣城.rAAV/BA46转染树突状细胞诱导特异细胞免疫[J].南方医科大学学报,2008,28(12):2146-2149. |
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| 作者姓名： | 尤长宣 苏瑾 廖旺军 张军一 Liu Yong Paul L Hermonat 罗荣城 |
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| 作者单位： | 南方医科大学南方医院肿瘤科,广东,广州,510515;南方医科大学南方医院呼吸科,广东,广州,510515;美国阿肯色大学医学院基因治疗中心,小石城,72205 |
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| 基金项目： | 中美合作研究基金
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广东省医学科学技术研究基金
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| 摘 要： | 目的 探讨以腺相关病毒为载体,乳腺癌抗原BA46基因转染树突状细胞(DC)诱导特异细胞免疫的可行性.方法 取健康人外周血,采用密度梯度离心法分离外周血单个核细胞(DC前体细胞),将细胞分为基冈转染组及对照组,基因转染组以重组腺相关病毒rAAV/BA46/Neo转染,对照组以293细胞裂解物冲击.两组细胞均采用重组人粒细胞巨噬细胞集落刺激因子(GM-CSF)、白细胞介素4(IL-4)、肿瘤坏死因子-α(TNF-α)诱导DC前体细胞成熟.第7天,收集DC,取该DC与T细胞按比例混合,诱导细胞毒性T淋巴细胞(CTL),用3H-TdR掺入法检测DC刺激自体淋巴细胞增殖能力;以流式细胞仪分析CTL细胞中IFN-γ、IL-4、CD4、CD8、CD25、CD69的表达情况,同时取BA46阳性的乳腺癌细胞株Hs578T为靶细胞,采用51Cr释放法检测杀伤效率.结果 BA46基凶转染组DC具备较强的刺激淋巴细胞增殖的能力,所诱导的CTL高表达CD8、CD69和IFN-γ,对BA46阳性的靶细胞有较强的杀伤作用,该杀伤具有BA46抗原特异性和MHC限制性.结论 乳腺癌BA46基因转染DC成功诱导特异的细胞免疫.为基因修饰细胞治疗乳腺癌打下实验室基础.
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| 关 键 词： | 乳腺癌 BA46 腺相关病毒 特异细胞免疫 |
rAAV/BA46-transfected dendritic cells can induce specific cellular immunity |
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| YOU Chang-xuan,SU Jin,LIAO Wang-jun,ZHANG Jun-yi,Liu Yong,Paul L Hermonat,LUO Rong-cheng.rAAV/BA46-transfected dendritic cells can induce specific cellular immunity[J].Journal of Southern Medical University,2008,28(12):2146-2149. |
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| Authors: | YOU Chang-xuan SU Jin LIAO Wang-jun ZHANG Jun-yi Liu Yong Paul L Hermonat LUO Rong-cheng |
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| Institution: | YOU Chang-xuan1,SU Jin2,LIAO Wang-jun1,ZHANG Jun-yi1,LIU Yong3,Paul L Hermonat3,LUO Rong-cheng1 1Department of Oncology,2Department of Respiration,Nanfang Hospital,Southern Medical University,Guangzhou 510515,China,3Gene Therapy Center,The University of Arkansas for Medical Science,Little Rock 72205,USA |
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| Abstract: | Objective To study the feasibility of transfecting breast cancer BA46 gene into dendritic cells (DCs) using adeno-associated virus (AAV) to induce specific cellular immunity. Methods Mononuclear cells (DC precursor) were isolated from the peripheral blood of healthy donors by density gradient centrifugation and infected with rAAV/BA46/Neo virus stock (transfection group) or pulsed with 293 cell lysate (control group). In both groups, maturation of the DC precursor was induced by granulocyte-macrophage colon... |
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| Keywords: | BA46 |
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