结肠癌组织ＨＭＧＢ１和ＶＥＧＦ-Ｄ的表达及其相关性

目的：探讨高迁移率族蛋白１（ＨＭＧＢ１）和血管内皮生长因子Ｄ（ＶＥＧＦ-Ｄ）在人结肠癌组织中的表达及其二者之间的相关性。方法：收集２００３～２００５年手术切除的７０例结肠癌组织标本，用免疫组织化学染色法检测结肠癌组织ＨＭＧＢ１和ＶＥＧＦ-Ｄ的表达情况，同时对两者行相关性分析。ＨＭＧＢ１作为干预因素，检测经ＨＭＧＢ１刺激后结肠癌细胞ＨＣＴ１１６中ＶＥＧＦ-ＤｍＲＮＡ表达的变化。结果：ＨＭＧＢ１在结肠癌组织中的阳性表达率为７２.９％，其中伴有淋巴结转移的表达率为８３３％，无淋巴结转移的表达率为５７.１％，两者差异有统计学意义。ＶＥＧＦ-Ｄ在结肠癌组织中的阳性表达率为３０％。ＨＭＧＢ１表达与ＶＥＧＦ-Ｄ表达呈正相关。结肠癌细胞ＨＣＴ１１６中ＶＥＧＦ-ＤｍＲＮＡ的表达受ＨＭＧＢ１的影响。结论：ＨＭＧＢ１可能通过诱导结肠癌组织中ＶＥＧＦ-Ｄ的表达，从而促进结肠癌的淋巴结转移。

The expressions and correlations of HMGB1 and VEGF-D in colon cancer

Objective：To discuss the correlation between the expressions of HMGB1 and VEGF-D in colon cancer.Methods：Seventy colon cancer samples were stained by immunohistochemical method for VEGF-D and HMGB1.The correlations of VEGF-D and HMGB1 were analyzed statistically.Colon cancer cell HCT116 was stimulated with HMGB1 and the changes of expression of VEGF-D were detected.Results：The positive rate of HMGB1 in lymph node metastasis group was higher significantly than that in negative lymph node group.Furthermore,the expression of HMGB1 was consistent with the expression of VEGF-D. The expression of VEGF-D mRNA in HCT116 was affected by HMGB1. Conclusion：HMGB1 can induce the expression of VEGF-D in colon cancer and promote the metastasis of colon cancer through lymphatics.