肾移植病人配组淋巴细胞毒抗体的检测

目的：通过对肾移植病人的配组淋巴细胞毒抗体（ｐａｎｅｌ ｒｅａｃｔｉｖｅ ａｎｔｉｂｏｄｙ ＰＲＡ）的检测，了解与分析肾移植病人配组淋巴细胞毒抗体的差异。方法：以３６名健康成人外周血淋巴细胞作为群体淋巴细胞来源，采用补体依赖微量淋巴细胞毒方法，检测病人血清中的配组淋巴细胞毒抗体。结果：５５例病人进行血清淋巴细胞毒交叉配合实验检测，只有１例阳性１．８２％。而ＰＲＡ检测８例病人血清为阳性（６女２男）占

The Detection of Panel Reactive Antibody in Patients to Receive Renal Transplantation

To investigate the possible difference in the positive rates of panel reactive antibody (PRA) among different patients to receive renal transplantation. Methods:The peripheral blood lymphocytes from 36 healthy adults were used in the study. The serum PRA in patients was detected by using the method of complement-dependent micro-lymphocytotoxicity test. Results:Lymphocyte samples from 55 cases of patients were subject to serum lymphocytotoxicity cross matching test,only one case was found positive (1.82%);while by PRA detection serum from 8 cases (6 female and 2 male) showed positive (14.5%). In these patients 35 cases had ever received blood transfusion,of them 6 cases were PRA-positive (17.2%),while of the 20 cases who had never received blood transfusion only 2 cases were PRA-positive (10%). Conclusion:There was difference in the positive rates of PRA between female and male patients ( P <0.05),while no difference was observed between patients having and not having received blood transfusion (P>0.05).Compared with serum lymphocytotoxicity cross test,the detection of PRA is more effective in accurately predicting the in vivo sensitization situation and antibody level in patients.