| 维生素D衍生物EB1089对胰腺癌细胞系BxPC-3生长的抑制作用 |
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| 引用本文： | 秦阳 郭克建. 维生素D衍生物EB1089对胰腺癌细胞系BxPC-3生长的抑制作用[J]. 胰腺病学, 2003, 3(3): 136-139 |
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| 作者姓名： | 秦阳 郭克建 |
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| 作者单位： | 中国医科大学附属第一医院外科 110001沈阳(秦阳)，中国医科大学附属第一医院外科 110001沈阳(郭克建) |
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| 摘 要： | 目的：观察维生素D衍生物EB1089对人胰腺癌细胞系BxPC-3的生长抑制作用及其可能机制。方法：应用MTT比色法、流式细胞术、Western blot法免疫印记电泳进行检测。结果：EB1089抑制人胰腺癌细胞系BxPC-3的生长，抑制50％细胞生长的药物浓度(IC50)为10^-7mmol／L。癌细胞在EB1089作用下，G0／G1期细胞比例增加23％，S期细胞比例下降12％。胰腺癌细胞系BxPC-3的p21蛋白的表达随EB1089的作用时间和药物浓度的提高而增强。结论：EB1089对胰腺癌细胞系：BxPC-3具有生长抑制作用，其作用机制可能与p21表达上调有关。
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| 关 键 词： | 维生素D衍生物 EB1089 胰腺癌 细胞系 BxPC-3生长 抑制作用 |
| 修稿时间： | 2003-06-09 |
Inhibitory effects of vitamin D analogues EB1089 on growth of human pancreatic cancer cell line BxPC-3 |
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| QIN Yang,GUO Kejian. Inhibitory effects of vitamin D analogues EB1089 on growth of human pancreatic cancer cell line BxPC-3[J]. Chinese JOurnal of Pancreatology, 2003, 3(3): 136-139 |
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| Authors: | QIN Yang GUO Kejian |
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| Affiliation: | QIN Yang,GUO Kejian. Department of Surgery,First Affiliated Hospital of China Medical University,Shenyang 110001,China |
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| Abstract: | Objective To observe and investigate inhibitory effects of EB1089 on the growth of BxPC-3 human pancreatic cancer cell line BxPC-3. Methods The antiproliferative effects of EB1089 on BxPC-3 were detected by MTT test. The changes of cell cycle were investigated flow cytometry by using. The levels of p21 expression were measured by Western blotting.Result EB1089 inhibited proliferation of the BxPC-3 cells. The proportion of cells in the G 0/G 1 phase increased and combined with the decrease of proportion of cells in the S phase. Conclusions The results suggest that EB1089 inhibits the growth of certain pancreatic cancer cells by up regulation of p21, which is therefore considered to be a potentially useful agent for new therapeutic strategies focusing on inhibition of pancreatic cancer cell proliferation. |
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| Keywords: | Pancreatic neoplasms Drug therapy Calcitriol Analogs and derivatives Therapeutic use |
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