MUC2反义脱氧寡核苷酸对胃癌细胞体外黏附侵袭活性的影响

目的 探讨黏蛋白MUC2反义脱氧寡核苷酸（ASODN）对人胃癌细胞株SGC7901黏附侵袭活性的影响。方法 采用人工合成的MUC2 ASODN经阳离子脂质体包裹后转染入SGC7901细胞中，采用黏附试验、Boyden小室体外侵袭试验观察比较转染前后癌细胞黏附率，穿膜细胞相对百分率及组织蛋白酶D、钙黏蛋白表达的变化。结果 转染SGC7901细胞48h后，癌细胞黏附率在30、60、90、120min各时间段逐渐升高，但低于空白对照组（P均〈0．01）；转染后癌细胞穿膜细胞相对百分率明显下降；转染后SGC7901细胞的E-钙黏蛋白表达明显增高，组织蛋白酶D水平明显降低。结论 人工合成的MUC2 ASODN能有效抑制胃癌细胞株SGC7901黏附侵袭能力。

Effect of MUC2 antisense oligodeoxynucleotideon on adherion and invasion ability of gastric cancer cells SGC7901 in vitro

[Objective] To investigate the inhibitory effect in vitro of mucin gene MUC2 antisense oligodeoxynucleotide(ASODN) on adherson and invasion ability of gastric cancer cells SGC7901.[Methods] Phosphorothioate MUC2 ASODN was synthesized and transfected to SGC7901 cells mediated by lipofectin.The inhibitory effects on cell adheson and invasion were determined by MTT method,Boyden chamber invasion assay and immunohistochemical method.[Results] 30,60,90,120 min after transfection,the adhesion ability increased gradually,but was lower compared with control cells(P<0.01).Data of Boyden chamber invasion assay indicaded the mean invasion percentage of SGC7901 was reduced compared with the control cells after ASODN transfected.MUC2 ASODN upregulated the expression of E-cadherin protein,downregulated the expression of cathepsin D.[Conclusion] In vitro,synthesized MUC2 ASODN can significantly inhibit adhesion and invasion ability of gastric cancer cells SGC7901.