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| 兔骨髓间充质干细胞复合两种材料体外生物相容性的比较 | |
| 引用本文： | 尹航,王晓东,张亚,张希峰,刘新晖,张锡庆,董寅生.兔骨髓间充质干细胞复合两种材料体外生物相容性的比较[J].中国矫形外科杂志,2006,14(20):1571-1573,I0003. |
| 作者姓名： | 尹航 王晓东 张亚 张希峰 刘新晖 张锡庆 董寅生 |
| 作者单位： | 1. 苏州大学附属儿童医院骨科,江苏,215003 2. 常熟市第二人民医院,江苏 3. 河北医科大学附属第一医院,石家庄 4. 东南大学材料科学与工程系,南京 |
| 基金项目： | 江苏省卫生厅135工程项目资助项目;苏州大学校科研和教改项目 |
| 摘 要： | 目的]探讨丝素蛋白和纳米晶羟基磷灰石胶原／骨体外生物相容性，为组织工程寻找理想的载体材料。方法]体外培养兔骨髓间充质干细胞分别于丝素蛋白和纳米晶羟基磷灰石胶原／骨体外复合培养，应用扫描电镜、激光共聚焦显微镜，四唑盐比色试验（MTT法），观察材料上复合培养的细胞的生物学特性。结果]兔骨髓间充质干细胞可以在丝素蛋白和纳米晶羟基磷灰石胶原／骨良好的黏附、增殖和生长。两种材料相比较其相容性没有明显的差异。结论]丝素蛋白和纳米晶羟基磷灰石胶原／骨均具有良好的生物相容性，可以作为组织工程理想的载体材料。 |
| 关 键 词： | 组织工程 细胞载体 丝素蛋白 纳米晶羟基磷灰石胶原 生物相容性 |
| 文章编号： | 1005-8478（2006）20-1571-03 |
| 收稿时间： | 2006-06-29 |
| 修稿时间： | 2006-06-29 |
Comparative study on the culture of rabbit bone marrow mesenchymal stems cells on two scaffold materials |
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| YIN Hang, WANG Xiao-dong, ZHANG Ya,et al..Comparative study on the culture of rabbit bone marrow mesenchymal stems cells on two scaffold materials[J].The Orthopedic Journal of China,2006,14(20):1571-1573,I0003. | |
| Authors: | YIN Hang WANG Xiao-dong ZHANG Ya |
| Institution: | YIN Hang, WANG Xiao-dong, ZHANG Ya, et al. |
| Abstract: | Objective]To explore the biocompability of silk fibroin(SF) or non-hydroxyapatite/collagen(NH/C) with bone marrow mesenchymal stem cells(BMMSCs) in order to find out a better scaffold material.Method]The third generation BMMSCs of rabbit were cultured on SF or NH/C and the biological characteristics of the cells were examined using scanning electronmicroscope,laser scan confocal microscope and MTT test.Result] The BMMSCs of rabbit grew and proliferated well on both SF and NH/C at 4-8 days cultures.There was no significant defference between the biocompability of BMMSCs on SF and on NH/C(P>0.05).Conclusion]Both SF and NH/C have good biocompability with BMMSCs and could be used as an ideal scaffold material in tissue engineering. |
| Keywords: | tissue engineering scaffold silk fibroin non-hydroxyapatite/collagen biocompatibility |
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