| 反相高效液相色谱法测定复肝宁片中绿原酸含量 |
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| 引用本文： | 杨芳,夏小叶,杨宝凡.反相高效液相色谱法测定复肝宁片中绿原酸含量[J].中国药业,2007,16(17):16-17. |
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| 作者姓名： | 杨芳 夏小叶 杨宝凡 |
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| 作者单位： | 1. 湖南省常德市药品检验所,湖南,常德,415101
2. 湖南德海制药有限公司,湖南,常德,415101 |
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| 摘 要： | 目的建立复肝宁片中绿原酸的含量测定方法。方法采用反相高效液相色谱（RP—HPLC）法，、色谱柱为Hypersil BDS C18柱（200mm×4.6mm，5μm），流动相为乙腈-0．4％磷酸溶液（13：87），流速1．0mL／min，检测波长为327nm，柱温30℃．结果绿原酸进样量线性范围为0.2～2．0μg（r=0．9995），平均加样回收率为97．98％，RSD为0．76％（n=6）。结论RP-HPLC法测定复肝宁片中绿原酸含量，方法可行，重现性好，可用于复肝宁片的质量控制。
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| 关 键 词： | 复肝宁片 绿原酸 反相高效液相色谱法 |
| 文章编号： | 1006-4931（2007）17-0016-02 |
| 修稿时间： | 2007-06-13 |
Determination of Chlorogenic Acid in Fuganning Tablets by RP- HPLC |
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| Yang Fang,Xia Xiaoye,Yang Baofan.Determination of Chlorogenic Acid in Fuganning Tablets by RP- HPLC[J].China Pharmaceuticals,2007,16(17):16-17. |
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| Authors: | Yang Fang Xia Xiaoye Yang Baofan |
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| Institution: | 1. Changde Institute for Drug Control of Hunan Province, Changde, Hunan, China 415101; 2. Hunan Dehai Pharmaceutical Co.. Ltd., Changde, Hunan, China 415101 |
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| Abstract: | Objective To establish a method for determination of chlorogenic acid in Fuganning tablets. Methods RP-HPLC was used with Hypersil BDS C18 （200 mm ×4.6 mm,5 μm） column. The mobile phase was acetonitrile-0.4% phosphoric aeid （13：87）. The detecting wavelength was 327 nm. The flow rate was 1.0 mL/min. The column temperature was 30℃. Results The calibration curves were linear within the range of 0. 2 - 2. 0 μg （ r = 0. 999 5） for Fuganning tablets. The average recovery rate was 97.98% with RSD = 0. 76% （ n = 6）. Conclusion The method is simple,reproducible and can be used for the quality control. |
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| Keywords: | Fuganning tablets chlorogenic acid RP-HPLC |
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