老年大鼠脑细胞内钙的变化

目的:观察老年大鼠脑细胞内钙浓度([Ca2 ]i)的变化.方法:以Fura/AM为荧光指示剂,采用荧光分光光度计测定急性分离大鼠脑[Ca2 ]i.结果:静息状态下老年大鼠脑[Ca2 ]i及对高钾除极化的反应均明显低于青年对照组(P＜0.01),但其增强作用在老年大鼠与青年大鼠间无明显差异(P＞0.05).结论:老年大鼠静息脑[Ca2 ]i降低,但在相同情况下老年大鼠脑细胞内钙超载更加明显,从而较青年大鼠更易发生脑细胞的损害.

Change of intracellular calcium in brain cells of aged rats

Objective: To observe the change of intracellular calcium([Ca~(2+)]i) in brain cells of aged rats.Methods:Fluorescence spectrophotometer with fura-2 as a calcium fluorant was used to measure [Ca~(2+)]i in acutely dissociated brain cells of aged rats.Results:In the presence of 1 mmol/L of extracellular Ca~(2+),resting [Ca~(2+)]i and the response of [Ca~(2+)]i to rise in KCl in brain cells of aged rats were all significantly reduced than those of young rats.But there was no significant difference in the increase of [Ca~(2+)]i induced by different concentrations of KCl [Ca~(2+)]i between the aged and the young rats(P>0.05).Conclusion:Resting [Ca~(2+)]i in brain cells of aged rats is reduced.The overoad of [Ca~(2+)]i in brain cells of aged rats is more significantly than that in young rats under same circumstances,so the brain cells of aged rats are more lirely to be damaged.