去窦弓神经大鼠心肌细胞凋亡及其相关基因蛋白表达的时程

目的:研究去窦弓神经（SAD）大鼠心肌细胞凋亡及其相关基因蛋白表达的时程。方法:10周龄SD大鼠行SAD术或假手术（Sham）,术后4、8、16和32周对SAD及相应Sham组大鼠的心肌连续切片,采用末端标记TUNEL法检测细胞凋亡;采用ABC法检测Bcl-2、Bax、Fas和Fas-L蛋白的表达,并用计算机图像分析技术进行观察和比较。结果:与Sham组相比,SAD大鼠心肌细胞凋亡率增加,Bcl-2表达减少,Bax、Fas、Fas-L表达增高,Bcl-2/Bax比率降低。结论:心肌细胞凋亡及其相关基因表达失调可能参与SAD大鼠的心肌重构,且具有一定的时间窗。

The Time Window of Myocardiocytes Apoptosis and Expression of Related Genesin Sinoaortic-denervated Rats

OBJECTIVE: To study the time window of apoptosis and related genes expression of the myocardiocytes in sinoaortic-denervated(SAD) rats.METHODS SAD of shamoperation(Sham) was performed in male SD rats at the age of 10 weeks.After 4, 8, 16, 32 weeks, apoptotic cells were stained in situ using terminal dexynucleotidyl - transferase mediated -dUTP nick end labeling(TUNEL) .All stained results were analysed by computer image analysis techniques.Bel-2, Bax, Fas and Fas-L were detected using immunohistochemical method.RESULTS: Compared with Sham controls, the number of apoptotic myocardiocytes was significantly increased;The expressions of Bel - 2 were significantly decreased, whereas Bax,Fas and Fas-L were significantly increased in SAD rats.The ratio of Bcl-2/ Bax was decreased.CONCLUSION Apoptosis and dysregulation of related gene expressions may be involved in the myocardial remodeling in SAD rats.