三七复方合剂对甲醛熏染大鼠睾丸组织雄激素受体和c-kit表达的影响

目的 通过检测三七复方合剂对甲醛熏染大鼠睾丸生精小管的形态学变化和睾丸组织雄激素受体(AR)和酪氨酸蛋白激酶受体c-kit的表达，验证三七复方合剂对慢性甲醛熏染的疗效。 方法 将18 只SD 雄性大鼠随机均分为对照组、甲醛组和治疗组，其中甲醛组和治疗组在甲醛熏染箱内熏染8周（8h/d），治疗组自第5周起每天给予三七复方合剂灌胃治疗，持续4周。实验结束时，所有大鼠称量体重和睾丸重量，组织切片行HE染色和免疫组织化学检测，观察睾丸生精小管的形态学变化及睾丸组织AR和c-kit的表达。 结果 甲醛组鼠重和睾丸重量明显下降；经三七复方合剂治疗后，鼠重和睾丸重量较甲醛组明显增加（P<0.05）。甲醛组大鼠的睾丸生精小管萎缩，小管上皮层细胞排列紊乱，精子明显减少；三七复方治疗4周后基本恢复正常，管腔内可见大量精子。甲醛组睾丸组织ＡＲ和酪氨酸蛋白激酶受体c-kit表达明显减弱，治疗组生精上皮细胞的雄激素受体和酪氨酸蛋白激酶受体c-kit表达明显增强（P<0.05），达到正常水平。 结论 较长时间甲醛熏染可使大鼠的体重和睾丸重量下降，生精小管损伤，睾丸组织中多种细胞AR和酪氨酸蛋白激酶受体c-kit的表达下降，三七复方合剂的应用有较明显治疗效果。

Effects of compound panax notoginseng on the expressions of androgen receptor and c-kit in testicular tissues in formaldehyde rats exposure

Objective To observe the morphological variation of seminiferous tubules and the expressions of the androgen receptor(AR) and tyrosine kinase receptor c-kit in the testicular tissue in formaldehyde exposure SD rats after being treated with compound panax notoginseng(CPN). Methods Eighteen male SD rats were randomly divided into three groups：control，formaldehyde and CPN group．Formaldehyde and CPN group were stayed in the chambers filled with formaldehyde vapor（69.5±8.3ppm）for 8 weeks（8hours/day）. From the fifth week, the rats in CPN group were treated with CPN(1ml/100g) everyday for 4 weeks. At the end of the experiment, the whole body and testes of all rats were weighed. The seminiferous tubules and the expressions of androgen receptor and c-kit in testicular tissues observed with HE staining and the immunohistochemical method,respectively. Results The weights of bodies and testes in the formaldehyde group decreased significantly in comparison with the control group.After treated with CPN for 4 weeks, both weights significantly increased compared to the formaldehyde group(EM>P/EM><0.05). The atrophy of the convoluted seminiferous tubules，disorderly arrangement, enlarged interval of epithelial cells of the tubules and the remarkable loss of the sperms were observed in the formaldehyde group using HE staining. The morphology of seminiferous tubules gradually recovered, and lots of spermatids appeared in the seminiferous tubules in CPN group.The expressions of androgen receptor and c-kit in testicular tissues decreased significantly in formaldehyde group, but increased remarkably almost to the normal level in CPN group(EM>P/EM><0.05). Conclusion Formaldehyde exposure for a long time causes the loss of the weights of bodies and testes and the declines of the expressions of androg