| A study on the integration of adeno－associated virus inverted terminal repeat fold structure in eukaryotic chromosome in vitr |
| |
| 引用本文： | 郭葆玉,胡宗林,徐慧敏,孔宪涛,陆德如. A study on the integration of adeno－associated virus inverted terminal repeat fold structure in eukaryotic chromosome in vitr[J]. 中国人民解放军军医大学学报, 1994, 0(4) |
| |
| 作者姓名： | 郭葆玉 胡宗林 徐慧敏 孔宪涛 陆德如 |
| |
| 作者单位： | Guo Baoyu; Hu Zonglin; Xu Huimin; Kong Xiantao;Lu Deru (Department of Molecular Genetics，Second Military Medical University，Shanghai 200433)(Department of Clinical Laboratory，Changzheng Hospital，Second Military Medical University，Shanghai 200003) |
| |
| 摘 要： | Ａｓｔｕｄｙｏｎｔｈｅｉｎｔｅｇｒａｔｉｏｎｏｆａｄｅｎｏ－ａｓｓｏｃｉａｔｅｄｖｉｒｕｓｉｎｖｅｒｔｅｄｔｅｒｍｉｎａｌｒｅｐｅａｔｆｏｌｄｓｔｒｕｃｔｕｒｅｉｎｅｕｋａｒｙｏｔｉｃｃｈｒｏｍｏｓｏｍｅｉｎｖｉｔｒｏＧｕｏＢａｏｙｕ（郭葆玉）；Ｈｕ...
|
A study on the integration of adeno-associated virus inverted terminal repeat fold structure in eukaryotic chromosome in vitro |
| |
| Guo Baoyu, Hu Zonglin, Xu Huimin, Kong Xiantao,Lu Deru. A study on the integration of adeno-associated virus inverted terminal repeat fold structure in eukaryotic chromosome in vitro[J]. Journal of Medical Colleges of PLA(China), 1994, 0(4) |
| |
| Authors: | Guo Baoyu Hu Zonglin Xu Huimin Kong Xiantao Lu Deru |
| |
| Abstract: | Adeno-associated virus(AAV) is a single-stranded linear defective parvovirus. Using the model proposed by Cavalier-Smith for replication of ss-DNA virus. the linear fragment of inverted terminal repeats(ITRs) from PAAV/neo was isolated, denatured and refolded over. The daughter DNA was synthesized by using 3'-OH terminus as a primer with the new strand covalently linked to the parental strand. After the palindromic structure with neomycin resistance gene controlled by SV40 early promoter into eukaryotic cells. Southern blot showed that the molecule of hairpin structure could facilitate the copies into mammalian cells in vitro. The experiment supports the Cavalier-Smith's replication model of ss-DNA virus and provides another new system for DNA transduction. |
| |
| Keywords: | adeno-associated virus end-fold-ITRs neomycin resistance gene gene transduction |
| 本文献已被 CNKI 等数据库收录！ |
