凝血酶受体在人牙髓组织和细胞中表达的免疫组化研究

目的：探讨凝血酶受体在人正常、炎症牙髓组织及体外培养的牙髓成纤维细胞中的表达及其意义。方法：体外培养人牙贿成纤维细胞，收集正常和炎症牙髓组织，采用免疫组织化学方法观察凝血酶受体在牙髓组织和细胞中的表达和分布。结果：体外培养的牙髓成纤维细胞可见受体强阳性表达，正常及炎症牙髓组织中成牙本质细胞层、牙髓细胞、血管内皮细胞均呈广泛的阳性表达，炎症区淋巴细胞、单核细胞、中性粒细胞染色为强阳性。结论：人牙髓组

Immunolocalization of thrombin receptor expressed in human dental pulp cell and tissue

AIM: To investigate the expression and distribution of thrombin receptor in human normal and inflamed dental pulp tissue and cultured dental pulp fibroblasts. METHODS: We applied immunohistochemical techniques to examine the thrombin receptor expression and distribution using an monoclonal antibody to human thrombin receptor. RESULTS: Cultured dental pulp fibroblasts were strong positive stained. Positive staining was observed in odontoblasts, dental pulp cells and vascular endothelial cells in both healthy and disease teeth. Strong specific staining was detected in lymphocytes, monocytes and neutrophils at sites of pulp inflammation. CONCLUTION: The expression of thrombin receptor in human dental pulp cell and tissue was detected, and this provides the basis for further studying the mechanism of thrombin in dental pulp.