高血压大鼠心脏和血管丝裂素活化蛋白激酶与其磷酸酶-1的表达

目的 :探讨MAPK与MKP 1在高血压心血管重塑中的可能作用。方法 :应用Western blot的方法观察了自发性高血压大鼠(SHR)及WKY大鼠心脏和血管丝裂素活化蛋白激酶及丝裂素活化蛋白激酶磷酸酶表达的改变。结果 :(1)WKY大鼠心脏中未见有丝裂素活化蛋白激酶的表达 ,而SHR心脏中检测到了丝裂素活化蛋白激酶的表达 ;并且SHR血管中丝裂素活化蛋白激酶的表达较WKY大鼠增强90 % (P <0 0 1) ;(2 )SHR心脏及血管中丝裂素活化蛋白激酶磷酸酶 - 1的表达较WKY大鼠分别降低 5 3%及 45 % (P均<0 0 1)。结论 :SHR心脏和血管重塑的发生除与丝裂素活化蛋白激酶表达增强有关外 ,可能还与丝裂素活化蛋白激酶磷酸酶 - 1的表达下降有关。

The Changes of Mitogen-Activated Protein Kinase and Mitogen-Activated Protein Kinase Phosphatase-1 in Cardiac and Vascular Smooth Muscles of Hypertensive Rats

Aim: The purpose of this study is to investigate the expression of mitogen activated protein kinase and mitogen activated protein kinase phosphatase 1 in cardiac and vascular smooth muscles of SHR. Methods:The expression of mitogen activated protein kinase and mitogen activated protein kinase phosphatase 1 were studied by Western blot. Results:The expression of mitogen activated protein kinase and mitogen activated protein kinase phosphatase 1 were found in cardiac muscles of SHR, but not in cardiac muscle of WKY rat. Meanwhile,the expression of mitogen activated protein kinase in vascular smooth muscles of SHR was 108% higher than that of WKY rat ( %P%< 0 01). Mitogen activated protein kinase phosphatase 1 expression in cardiac and vascular smooth muscles of SHR was marked decreased by 53% and 45% (all %P%< 0 01), compared with WKY rat. Conclusions:The results suggested that the remodelling of cardiac and vascular smooth muscles in SHR might be related to the increased expression of mitogen activated protein kinase and the decreased expression of mitogen activated protein kinase phosphatase 1.