同系与异系移植MMPs、TIMPs表达的比较实验研究

目的：动态观察MMPs、TIMPs在移植动脉中的表达规律，探讨其在移植动脉硬化中的作用。方法：将Wistar大鼠120只、SD大鼠40只随机分为10组，异系（SD-Wistar）移植组和同系（Wistar-Wistar）移植组各5组，分别于术后3d和1、2、4、8w采取。形态学观察、免疫组织化学SP染色法测定移植段腹主动脉内MMP-1、TIMP-1，3的表达、Northern杂交定量分析MMP-2表达，并加以比较。结果：①术后2～8w异系移植组移植段腹主动脉内膜厚度较同系移植组显著增生（P〈0．05）。②同系移植组MMP-1，2和TIMP－1较低，峰值均出现于术后2～4w；异系移植组表达显著增加，MMP-1，2峰值出现于术后第2w，TIMP-1峰值出现于术后第4w。③TIMP－3在各组中表达均较低，仅见于细胞外基质中。结论：移植时慢性排斥反应过程中MMPs/TIMPs表达时象及量效的不平衡导致了细胞外基质合成与降解的失衡，是移植物的动脉硬化发生的重要原因。

Investigation of the expressions of MMPs and TIMPs in rat transplantation arteriosclerosis model of Chronic Rejection

Objective:To dynamic observe the rule of the expressions of MMP-s and TIMP-s on the transplanted artery,which is for discussing these functions of transplanted arteriosclerosis.Methods:The expressions of MMP-s and TIMP-s in the tissues of transplanted abdominal aorta were mensurated by morphology,immunohistochemical SP staining method and the expression of MMP-2 was detected by Northern blot quantitative analysis in abdominal aortic orthotopic transplantation model.Results:The thickness of tunica intima of the allografts was much evidently thicker than isografts 2 weeks after transplantation(P<0.05).The expressions of MMP-s and TIMP-s in the isografts came to the highest on 14th day and then declined.But the expressions of MMP-s and TIMP-s in the allografts were observably higher tgan the former,and the peak of the expression of TIMP-1 was prolonged to 4th week.TIMP-3 kept lowly expressed in every groups.Conclusion:The unbalance of expressions of MMP-s/TIMP-s leading to the turbulence of synthesization and degradation ECM,is an important reason for the process of chronic rejection.