PI3-Kinase-dependent electrogenic intestinal transport of glucose and amino acids |
| |
Authors: | Rexhep Rexhepaj Ferruh Artunc Marco Metzger Thomas Skutella Florian Lang |
| |
Institution: | (1) Department of Physiology I, University of Tübingen, Gmelinstr. 5, 72076 Tübingen, Germany;(2) Department of Anatomy, University of Tübingen, Tübingen, Germany |
| |
Abstract: | Intestinal glucose and amino acid transport is stimulated by the serum- and glucocorticoid-inducible kinase isoforms SGK1,
SGK2, and SGK3 and protein kinase B which are, in turn, stimulated following activation of the phosphoinositol-3 kinase (PI3
kinase). The present study has been performed to explore whether pharmacological inhibition of the PI3 kinase affects electrogenic
jejunal transport of glucose and amino acids. In Ussing chamber experiments, glucose (20 mM), phenylalanine (20 mM), glutamine
(20 mM), cysteine (20 mM), and proline (20 mM) generated lumen negative currents (I
glc, I
phe, I
gln, I
cys, and I
pro), respectively, which gradually declined following application of the PI3 kinase inhibitor Wortmannin (1 μM). Within 40 min,
Wortmannin treatment significantly decreased I
glc by 39 ± 10% (n = 5), I
phe by 70 ± 7% (n = 4), I
gln by 69 ± 8% (n = 4), I
cys by 67 ± 8% (n = 6), and I
prol by 79 ± 12% (n = 3). A similar decline of I
glc was observed following application of the PI3 kinase inhibitor LY294002 (50 μM). Exposure to the inhibitors did not significantly
alter transepithelial potential difference and resistance in the absence of substrates for electrogenic transport. The observations
suggest that the electrogenic transport of glucose and several amino acids requires the continued activity of PI3 kinase.
R. Rexhepaj and F. Artunc shared first authorship. |
| |
Keywords: | PI3 kinase Wortmannin Intestine Phenylalanine Glutamine Cysteine Proline |
本文献已被 PubMed SpringerLink 等数据库收录! |
|