P选择素在高胆固醇血症小鼠肾脏的表达

目的:研究P选择素在高胆固醇血症小鼠肾脏的表达,探讨脂质肾毒性损伤的机制。方法:24只雌性C57/BL小鼠分为2组,普通膳食组(A组)和高胆固醇膳食组(B组),喂养15周,用生化酶法检测血脂;用PAS和HE染色观察肾脏组织学改变;免疫组化SABC法检测P选择素的表达。结果:B组血清总胆固醇(Tch)和低密度脂蛋白胆固醇(LDL-C)浓度显著高于A组(P<0.05,P<0.01);肾小球细胞数及系膜区占肾小球面积比显著高于A组(P<0.05,P<0.01);P选择素表达明显增加(P<0.01)。结论:P选择素的明显表达可能与脂质引起的肾小管间质损害有关。

Expression of P-selectin in kidney of mice with hypercholesterolemia

Objective: To detect the expression of P-selectin in mice with hypercholesterolemia,and to explore the mechanism of toxic injury to the kidney induced by lipid. Methods: Twenty-four C57/BL mice were divided into 2 groups: group A (regular diet) and group B (hypercholesterol diet). After 15 weeks,the level of blood lipid was detected by enzymatic method. The histological changes of the kidney were observed after PAS and HE staining. The expression of P-selectin was determined by streptavidin-biotin complex (SABC) method. Results: The levels of serum total cholesterol and low-density lipoprotein cholesterol in group B were significantly higher than those in group A (P < 0.05 and P < 0.01). Compared with group A,the glomerular cell number,mesangial area,and expression of P-selectin significantly increased in group B (P < 0.05,P < 0.01,and P < 0.01). Conclusion: P-selectin may be involved in the renal tubular-interstitial injury induced by lipid.