| BAG-1、Bcl-2双靶区反义RNA重组载体诱导SGC-7901细胞凋亡 |
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| 引用本文： | 陈建国,卢天龙,韩跃武,冯惟萍,任慧子. BAG-1、Bcl-2双靶区反义RNA重组载体诱导SGC-7901细胞凋亡[J]. 第三军医大学学报, 2009, 31(2): 132-135 |
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| 作者姓名： | 陈建国 卢天龙 韩跃武 冯惟萍 任慧子 |
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| 作者单位： | 兰州大学基础医学院医学生物化学与分子生物学研究所,兰州,730000;甘肃省卫生学校,兰州,730000;兰州大学基础医学院医学生物化学与分子生物学研究所,兰州,730000 |
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| 摘 要： | 目的 构建BAG-1、Bcl-2双靶区反义RNA重组载体并初步探讨其对SGC-7901细胞增殖活性的影响.方法 从SGC-7901细胞总RNA中逆转录扩增包括全部编码序列的BAG-1和Bcl-2 cDNA,利用生物工程技术,反向插入真核细胞双表达载体pVITR02的多克隆位点中,转染SGC-7901细胞,MTT法检测对细胞增殖的影响,RT-PCR法观察对细胞BAG-1和Bcl-2 mRNA表达水平的变化,流式细胞术检测对细胞周期的影响.结果 限制性内切酶和测序分析表明,双表达质粒pVlTR02-AsBAG-1-Bcl-2构建成功.MTT法检测显示pVITR02-AsBAG-1-Bcl-2载体抑制SGC-7901细胞增殖,并旱时间依赖性,其中以72 h转染组抑制作用最显著(P＜0.01);与对照组比较,pVITR02-AsBAG-1-Bcl-2组BAG-1和Bcl-2mRNA表达水平下降(P＜0.05),细胞捌亡比例升高(P＜0.01).结论 成功构建反义双靶区重组载体pVITR02-AsBAG-1-Bcl-2,并发现其能抑制SGC-7901细胞增殖并引起细胞凋亡.
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| 关 键 词： | BAG.1 Bcl-2 双表达质粒 反义RNA 胃癌 |
Recombinant vector carrying antisense RNA to dual-target BAG-1 and Bcl-2 induces SGC-7901 cell apoptosis |
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| CHEN Jian-guo,LU Tian-long,HAN Yue-wu,FENG Wei-ping,REN Hui-zi. Recombinant vector carrying antisense RNA to dual-target BAG-1 and Bcl-2 induces SGC-7901 cell apoptosis[J]. Acta Academiae Medicinae Militaris Tertiae, 2009, 31(2): 132-135 |
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| Authors: | CHEN Jian-guo LU Tian-long HAN Yue-wu FENG Wei-ping REN Hui-zi |
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| Affiliation: | CHEN Jian-guo1,2,LU Tian-long1,HAN Yue-wu1,FENG Wei-ping1,REN Hui-zi1(1Department of Biochemistry and Molecular Biology,School of Basic Medical Sciences,Lanzhou University,Lanzhou 730000,2Gansu Health School,China) |
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| Abstract: | Objective To construct the recombinant co-expression vector carrying antisense RNAs to dual-target BAG-1 and Bcl-2 and investigate its effect on the proliferation of SGC-7901 cells.Methods RT-PCR was used to amplify the full length CDS of BAG-1 and Bcl-2 cDNA from total RNA of SGC-7901 cells.The cDNA fragments of BAG-1 and Bcl-2 were inserted into eukaryotic co-expression pVITRO2 vector multiple cloning sites in the antisense orientation respectively by gene recombination technology.Then the recombinant pla... |
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| Keywords: | BAG.1 Bcl-2 |
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