转录因子FOXR2对脑胶质瘤细胞增殖的影响

目的探讨下调或过表达FOXR2对脑胶质瘤细胞增殖能力的影响。方法以正常脑组织标本为模板,PCR法克隆FOXR2基因,并构建FOXR2过表达质粒;Western blot法检测FOXR2在胶质瘤细胞系中的表达水平。用慢病毒系统构建稳定下调及过表达FOXR2的脑胶质瘤细胞株;CCK8实验检测下调或过表达FOXR2对胶质瘤细胞增殖速率的影响。结果成功克隆FOXR2基因;WB检测结果显示稳定下调FOXR2的U251细胞株及过表达FOXR2的U87细胞株构建成功。CCK8实验结果表明,与对照组相比,96 h后下调FOXR2的胶质瘤细胞增殖速率减少45.13%;相反,过表达FOXR2后,胶质瘤细胞的增殖速率可增加43.98%。结论转录因子FOXR2可促进胶质瘤细胞的增殖。

Effects of FOXR2 on proliferation of glioma cells

Objective To evaluate the effects of FOXR2 on proliferation of glioma cells. Methods The FOXR2 gene were cloned by PCR using cDNA of nontumorous tissue as template and constructed over-expression FOXR2 plasmid; Western blot assay was used to detect the levels of FOXR2 in glioma cell lines.We also constructed stable cell lines of down-regulation and over-expression FOXR2 using lentivirus vector system.CCK8 assay was used to assess the effects of FOXR2 on glioma cell proliferation.Results FOXR2 gene was cloned, and the stable glioma cell lines of down-regulation and over-expression FOXR2 were established successfully;The results of CCK8 assay showed that compared with control group, the cell proliferative rate decreased by 45.13%in FOXR2 down-regulation group, while, the cell number increased by 43.98% in FOXR2 over-expression group.Conclusion FOXR2 could significantly promote the proliferation ability of glioma cells .