| Abstract: | Cells transformed by Moloney sarcoma virus (MSV) take up 2-deoxyglucose at a faster rate at 39° C than uninfected or Moloney leukemia virus (MoLV)—infected normal rat kidney (NRK) cells. In a sarcoma-virus-transformed cell line, NRK (MSV-lb), whose transformed phenotype is expressed at 39° C (permissive) but not at 33° C (non-permissive), the stimulation of 2-deoxyglucose uptake at 39° C is temperature dependent; the increase is observed when cells are grown at 39° C but not at 33 C. When these cells were shifted from the non-permissive to the permissive temperature, the uptake increased from a rate near that of uninfected cells to a rate half that of NRK cells infected with Moloney sarcoma-leukemia complex (MSV-MoLV). The reverse change occurred when the cells were shifted from the permissive to the non-permissive temperature. Thus in a sarcoma virus-infected rat cell line where the maintenance of the transformed state is dependent on a cold-sensitive viral function, the uptake of 2-deoxyglucose is reduced in the cold-sensitive cells and correlated with the expression of the transformed phenotype. |
