| 弓形虫速殖子和包囊的分离纯化 |
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| 引用本文： | 沈继龙,龟井喜世子. 弓形虫速殖子和包囊的分离纯化[J]. 蚌埠医学院学报, 1991, 0(4) |
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| 作者姓名： | 沈继龙 龟井喜世子 |
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| 作者单位： | 蚌埠医学院寄生虫学教研室(沈继龙)，日本帝京大学医学部寄生虫学教研室(龟井喜世子) |
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| 摘 要： | 采用植物血凝素P(PHAP)亲和凝集分离法自小鼠腹腔液内分离弓形虫速殖子,纯度达99.5％,收获率为73.2％。与胰蛋白酶消化法相比,前法对虫体无损伤。此外,应用淋巴细胞分离介质Ficoll-Paque密度梯度离心法自鼠脑组织内分离出较纯净的包囊。
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| 关 键 词： | 弓形虫 速殖子 包囊 纯化 |
SEPARATION AND PURIFICATION OF TOXOPLASMA TACHYZOITES AND CYSTS FROM MOUSE HOST CELLS |
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| Shen Jilong,Kemei K. SEPARATION AND PURIFICATION OF TOXOPLASMA TACHYZOITES AND CYSTS FROM MOUSE HOST CELLS[J]. Journal of Bengbu Medical College, 1991, 0(4) |
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| Authors: | Shen Jilong Kemei K |
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| Abstract: | Toxoplasma gondii tachyzoites were separated from mouse peritoncal exudates by using a lectin of phytohemagglutinin P. A purity of 99.5% and a recovery of 73.2% were obtained and no chemicaldamage to the parasite surface protein was found when compared with trypsin digestion procedure by SDS-polyacrylamide gel clectro-phoresis. Besides, cysts were isolated from mouse brain tissues with density-gradient centrifugation on Ficoll-Paque, a lymphocyte isolation liquid. |
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| Keywords: | Toxoplasma tachyzoite cyst purification |
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