清肺止哮汤对哮喘小鼠气道炎症抑制作用的实验研究

目的:探讨中药复方清肺止哮汤对哮喘小鼠气道炎症的影响.方法:用卵白蛋白(OVA)致敏、雾化建立小鼠哮喘模型.末次抗原激发后48h收集支气管肺泡灌洗液(BALF)和骨髓标本,计数细胞总数和嗜酸性粒细胞(Eos),并行肺组织学检查.结果:清肺止哮汤抑制气道炎症的最合适剂量为2.5g/ml.清肺止哮汤可明显减少BALF中细胞总数(66.66±6.42)×104]及Eos绝对数(3.52±0.53)×104],能使肺组织中细支气管、血管周围的Eos浸润和粘液的过度分泌得到控制.但和布地奈德(BUD)组(56.00±9.28)×104,(0.86±0.26)×104]相比仍有显著性差异.结论:清肺止哮汤能部分抑制哮喘小鼠气道炎症.

The Chinese Herbal Medicine Formula Qing Fei Zhi Xiao Tang Suppresses Allergic Airway Inflammation in a Murine Model of Asthma

Objective:To investigate the effects of the Chinese herbal medicine formula Qing Fei Zhi Xiao Tang on inhibiting airway inflammation in a murine model of asthma.Methods:C57BL/6 mice were sensitized intraperitoneally and challenged aerosolized with ovalbumin(OVA)to establish an asthmatic model. Qing Fei Zhi Xiao Tang according to the corresponding dose was administered intragastrically after being firstly sensitized.48h after the last challenge collect bronchoalveolar lavage fluid(BALF) and bone marrow cell suspension,cell counts and differentials.Then the lungs were fixed in neutral buffered formaldehyde.Three micro meter paraffin sections were stained with hematoxylin and PAS and examined microscopically.Results:An optimal dose of Qing Fei Zhi Xiao Tang 2 5g/ml for 26 days was used in this study,which was the dose to inhibit inflammation of the airways.The total number of cells and the number of eosinophils (Eos) in BALF were decreased significantly,compared with those in OVA group,but there was statistically significant difference between Qing Fei Zhi Xiao Tang and BUD treatment.Lung histologic examination also demonstrated that Qing Fei Zhi Xiao Tang reduced inflammation and mucus hypersecretion.Conclusion:The Chinese herbal medicine formula Qing Fei Zhi Xiao Tang can partially inhibit allergen induced airway inflammation in a well characterized murine model of asthma.