Lipocalin型前列腺素D合成酶在大鼠睾丸和附睾中的分布与定位

目的 分析大鼠睾丸和附睾中Lipocalin型前列腺素D合成酶(LPGDS)的分布与定位，探讨LPGDS在男性生殖中的作用。方法 选用2月龄SD大鼠睾丸与附睾，运用Western blot方法分析LPGDS在大鼠睾丸与附睾头、附睾体和附睾尾中的表达及其差异；运用免疫组织化学方法分析LPGDS在睾丸与附睾头、附睾体和附睾尾中的分布。结果 Western blot研究发现，LPGDS在大鼠睾丸中没有表达，在附睾中有较强表达，附睾头表达量最高，附睾体次之．附睾尾最低。免疫组织化学结果显示，免疫阳性产物定位于附睾上皮细胞的胞质和纤毛上。结论 LPGDS在大鼠睾丸中没有表达，在附睾中有较强表达，表达量存在差异。提示，LPGDS在精子成熟过程中起着一定的作用。

DISTRIBUTION AND LOCALIZATION OF LIPOCALIN-TYPE PROSTAGLANDIN D SYNTHASE IN RAT TESTIS AND EPIDIDYMIDIS

Objective To explore the distribution and localization of LPGDS (Lipocalin-type prostaglandin D synthase) in rat testis and epididymidis and gain further insight into LPGDS potential function in male reproduction. Methods The expression of LPGDS in rat testis and epididymidis was assessed using RT-PCR and immunobloting. Using immunohistochemistry to explore the distribution of LPGDS in rat testis and epididymidis. Results None of the LPGDS studied was present in the testis of 2 month old SD rat. LPGDS was strikingly expressed in the caput epididymidis, while moderate to weak expressing was observed in the corpus and cauda epididymidis. The result of immunohistochemistry displayed that LPGDS was mainly localized within the cytoplasm and the cilia of the epithelial cells in rat epididymidis. Conclusion LPGDS was mainly distributed in epididymidis and localized with in the cytoplasm and the cilia of the epithelial cells. There was no LPGDS expressed in the testis of 2 month old SD rat, but it was strikingly expressed in the epididymidis, and the dose of expression was different, which suggested that LPGDS may play some kind of role during the process of the spermatozoa maturation.