A simple high-performance liquid chromatographic method for detection of hydroxyzine in human plasma after overdose |
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Authors: | Péhourcq Fabienne |
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Institution: | Department of Clinical Pharmacology and Toxicology, CHU Pellegrin, Place Amélie Raba-Léon, 33076 Bordeaux Cedex, France. fabienne.pehourcq@chu-bordeaux.fr |
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Abstract: | INTRODUCTION: Hydroxyzine, a piperazine H1-receptor antagonist, is effective in generalised anxiety disorder. For toxicological purposes, a simple reversed-phase high-performance liquid chromatographic assay was developed for the detection of hydroxyzine in human plasma. METHODS: A liquid-liquid procedure was used to extract the drug from plasma in the presence of an internal standard (clothiapine). The analysis was performed on a Spherisorb S5 C(8) analytical column with UV detection. RESULTS: A linear response was observed over the concentration range 20-1500 ng/ml. A good accuracy (bias<7.3%) was achieved for all quality controls, with intraday and interday variation coefficients inferior to 8.5%. The limit of detection was 10 ng/ml, without interference of endogenous components. DISCUSSION: This rapid method (run time<13 min) is currently used for poison management involving hydroxyzine. |
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