| 纳洛酮对大鼠缺血再灌注心肌细胞凋亡及Fas?FasL表达的影响 |
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| 引用本文： | 周维东,夏安周,张 探,李 娜,沙兴志,丁义勋. 纳洛酮对大鼠缺血再灌注心肌细胞凋亡及Fas?FasL表达的影响[J]. 南京医科大学学报(自然科学版), 2011, 0(3): 395-398 |
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| 作者姓名： | 周维东 夏安周 张 探 李 娜 沙兴志 丁义勋 |
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| 作者单位： | 睢宁县人民医院心内科;徐州医学院药理教研室; |
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| 基金项目： | 徐州市科技局基金资助(XM08C117) |
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| 摘 要： | 目的:观察纳洛酮(naloxone,Nal)对大鼠缺血再灌注心肌细胞凋亡及Fas、FasL蛋白表达的影响并探讨其机制。方法:SD大鼠24只,随机分成假手术组(Sham组)、缺血再灌注组(IR组)和纳洛酮处理组(Nal组)。原位末端标记法(TUNEL)检测心肌细胞凋亡情况;蛋白免疫印迹法(Western blotting)检测Fas、FasL蛋白表达;光镜下观察心肌组织病理学的改变。结果:与Sham组相比,IR组可见大量心肌细胞凋亡,Fas、FasL蛋白表达显著增加(P<0.01)。与IR组相比,Nal组心肌细胞凋亡显著减少,Fas、FasL蛋白表达明显降低(P<0.01)。IR组见心肌组织呈大小不等的灶性坏死,Nal组心肌坏死不明显。结论:心肌缺血再灌注时Fas、FasL表达介导心肌细胞凋亡,纳洛酮通过抑制Fas、FasL蛋白的表达而减少细胞凋亡从而起到保护心肌的作用。
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| 关 键 词： | 缺血再灌注 细胞凋亡 Fas蛋白 FasL蛋白 纳洛酮 |
| 收稿时间： | 2010-08-17 |
Effect of naloxone on apoptosis and expression of Fas and FasL protein in rats with myocardial ischemia-reperfusion injury |
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| ZHOU Wei-dong,XIA An-zhou,ZHANG Tan,LI N,SHA Xing-zhi and DING Yi-xun. Effect of naloxone on apoptosis and expression of Fas and FasL protein in rats with myocardial ischemia-reperfusion injury[J]. Acta Universitatis Medicinalis Nanjing, 2011, 0(3): 395-398 |
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| Authors: | ZHOU Wei-dong XIA An-zhou ZHANG Tan LI N SHA Xing-zhi DING Yi-xun |
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| Affiliation: | ZHOU Wei-dong,XIA An-zhou1*,ZHANG Tan1,LI Na1,SHA Xing-zhi,DING Yi-xun(Department of Cardiology,Suining Hospital,Suining 221200,1Department of Pharmacology,Xuzhou Medical College,Xuzhou 221004,China) |
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| Abstract: | Objective:To observe the effect of naloxone(Nal) on cardiomyocyte apoptosis and expression of Fas and FasL following acute ischemia-reperfusion injury in vivo and to explore its mechanism.Methods:Twenty-four SD rats were selected and randomly divided into three groups:sham-operated group(sham group),ischemia-reperfusion group(IR group),IR and treated with naloxone group(Nal group).Terminal deoxynucleotidyl transferase mediated dUTP nick end labeling(TUNEL) and Western blotting were used respectively to dete... |
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| Keywords: | ischemia-reperfusion apoptosis Fas FasL naloxone |
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