| FISH检测hTERC基因在宫颈腺癌中的扩增及意义 |
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| 引用本文： | 顾 芸,武卫平,钱 宁,冯振卿. FISH检测hTERC基因在宫颈腺癌中的扩增及意义[J]. 南京医科大学学报(自然科学版), 2011, 0(8): 1148-1151 |
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| 作者姓名： | 顾 芸 武卫平 钱 宁 冯振卿 |
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| 作者单位： | 南京医科大学附属南京妇幼保健院病理科;南京医科大学病理学系; |
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| 基金项目： | 国家“973”计划资助课题(2010CB933902) |
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| 摘 要： | 目的:探讨人端粒酶RNA(hTERC)基因在宫颈腺癌中的扩增以及在宫颈腺癌诊断和预后评估中的价值。方法:收集南京医科大学附属南京妇幼保健院2006年1月～2009年12月诊断为宫颈腺癌的石蜡标本38例,并随机抽取同时期诊断为宫颈鳞状上皮内瘤变而腺体正常的37例标本作为对照,采用荧光原位杂交(FISH)方法检测hTERC基因在腺癌及对照组宫颈腺体中的扩增。结果:腺癌组hTERC基因扩增阳性率为84.21%(32/38),对照组阳性率为0%(0/37),两组差异有显著统计学意义(P<0.01)。hTERC基因扩增检测的灵敏度为84.21%(32/38),特异度为100%(37/37),阳性预测值为100%(32/32),阴性预测值为86.05%(37/43)。FISH检测结果显示,不同亚型的宫颈腺癌阳性检出率各不相同,高分化和中-低分化的宮颈腺癌hTERC基因扩增程度存在差异(P<0.05)。对照组杂交信号多为2∶2,而腺癌组异常扩增类型有2∶3、2∶4、2∶5、3∶3、4∶4及N∶N。不同hTERC基因扩增程度的腺癌预后结果差异无统计学意义(P>0.05)。结论:hTERC基因在宫颈腺癌中扩增,病理分化程度不同...
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| 关 键 词： | 宫颈腺癌 人端粒酶 荧光原位杂交 |
| 收稿时间： | 2011-04-27 |
Amplification and significance of the hTERC gene detected by FISH in cervical adenocarcinoma |
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| GU Yun,WU Wei-ping,QIAN Ning and FENG Zhen-qing. Amplification and significance of the hTERC gene detected by FISH in cervical adenocarcinoma[J]. Acta Universitatis Medicinalis Nanjing, 2011, 0(8): 1148-1151 |
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| Authors: | GU Yun WU Wei-ping QIAN Ning FENG Zhen-qing |
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| Affiliation: | GU Yun,WU Wei-ping,QIAN Ning,FENG Zhen-qing 1(Department of Pathology,Nanjing Maternal and Child Health Hospital Affiliated to NJMU,Nanjing 210004,1 Department of Pathology,NJMU,Nanjing 210029,China) |
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| Abstract: | Objective:To investigate the amplification of the hTERC gene in cervical adenocarcinomas(CA) and its role in pathologic diagnosis and prognostic evaluation.Methods:A total of 38 paraffin-embedded samples were collected from patients diagnosed as CA in Nanjing Maternity and Child Health Hospital Affiliated to NJMU from Jan,2006 to Dec,2009,and 37 samples diagnosed cervical intraepithelial neoplasia(CIN) with normal cervical gland were severed as control group.These samples were analyzed using fluorescence in... |
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| Keywords: | cervical adenocarcinoma hTERC fluorescence in situ hybridization |
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