| 异种骨髓间充质干细胞胸腺修饰诱导免疫耐受的可行性研究 |
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| 引用本文： | 翟光地,沈振亚. 异种骨髓间充质干细胞胸腺修饰诱导免疫耐受的可行性研究[J]. 南京医科大学学报(自然科学版), 2011, 0(4): 474-477 |
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| 作者姓名： | 翟光地 沈振亚 |
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| 作者单位： | 南京医科大学附属常州二院心胸外科;苏州大学附属第一医院心血管外科和器官移植研究室; |
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| 摘 要： | 目的:确定骨髓间充质干细胞(BMSCs)在异种胸腺内的演化过程,为进一步进行BMSCs胸腺修饰诱导异种免疫耐受的研究提供可行性依据.方法:取豚鼠BMSCs经分离纯化体外扩增至第3代,并行胎牛血清-Fe2O3和4,6-二咪基-4-联苯基吲哚(DAPI)标记,标记后的豚鼠BMSCs行胸腺注射,7天后进行切片普鲁士蓝-中性红...
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| 关 键 词： | 胸腺修饰 骨髓间充质干细胞 胎牛血清-Fe2O3 免疫耐受 |
| 收稿时间： | 2010-08-14 |
immunotolerance induced by intrathymic inoculation of xenogeneic bone mesenchymal stem cells |
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| zhai guang-di and shen zhen-ya. immunotolerance induced by intrathymic inoculation of xenogeneic bone mesenchymal stem cells[J]. Acta Universitatis Medicinalis Nanjing, 2011, 0(4): 474-477 |
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| Authors: | zhai guang-di and shen zhen-ya |
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| Affiliation: | ZHAI Guang-di,SHEN Zhen-ya1(Department of Thoracic Surgery,Changzhou No 2 People's Hospital Affiliated to NJMU,Changzhou 213003,1Department of Cardiovascular Surgery,the First Affiliated Hospital of Suzhou University,Suzhou 215006,China) |
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| Abstract: | Objective:To observe the proliferation and differentiation of xenogeneic bone mesenchymal stem cells(BMSCs) in the thymus and to explore the feasibility of intrathymic inoculation with xenograft-BMSCs in inducing immunotolerance.Methods:BMSCs of guinea pigs were cultured in medium to P3,and labeled with fetal bovine serum-Fe2O3 and 4,6-diamidino-2-phenylindole(DAPI).The marked BMSCs were inoculated into thymuses gland of rats.The thymuses were sliced up and dyed with perls solution seven days later and obse... |
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| Keywords: | intrathymic inoculation BMSCs fetal bovine serum-Fe2O3 immunotolerance |
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