小鼠骨髓来源树突状细胞培养鉴定和诱导T淋巴细胞增殖

目的:建立一种体外诱导培养小鼠树突状细胞(DCs)的方法,并观察其不同生长阶段生物学形态及刺激淋巴细胞增殖的能力。方法:联合应用粒细胞-巨噬细胞集落刺激因子(GM-CSF)、白细胞介素-4(IL-4)诱导培养小鼠骨髓单个核细胞向DCs分化,并从形态学、表型及功能方面对其加以检测。结果:用GM-CSF和IL-4诱导培养小鼠骨髓来源的单核细胞,3 d后可见细胞形态改变,并呈集簇生长,培养至5 d可见典型的树突状突起,体外诱导培养8 d后获得大量成熟的DCs,扫描电镜可见典型的树突状细胞形态,高表达骨髓来源DCs特异性标记CD11c和共刺激分子CD40、CD80、CD86和MHC-Ⅱ,成熟DC具有很强的刺激同基因型和同种异基因型T淋巴细胞增殖的能力。结论:小鼠骨髓来源的单个核细胞体外诱导培养可生成大量成熟的DCs,为进一步抗肿瘤疫苗的实验研究奠定了基础。

Culture and identification of mouse bone marrow derived DCs that induce T lymphocyte proliferation

Objective:To establish a method of inducing and culturing dendritic cells from mouse bone marrow in vitro,and observe their biological feature and function of stimulating T lymphocytes proliferate in different stages.Methods:Mononuclear cells isolated from mouse bone marrow were induced into dendritic cells by being cultured with GM-CSF and IL-4,and then examined from aspects of morphology,phenotype and function.Results:Modality of mononuclear cells diversified after being cultured in GM-CSF and IL-4 for 3 ...