首乌延寿丹对大鼠局灶性脑缺血的保护作用

目的研究首乌延寿丹对大鼠局灶性脑缺血的保护机制。方法采用改良的线栓法制作大鼠大脑中动脉栓塞模型(MCAO)。将健康成年大鼠随机分为5组:假手术组、缺血模型组、缺血前首乌延寿丹低、中、高处理组。测定丙二醛(MDA)含量、超氧化物歧化酶(SOD)、过氧化氢酶(CAT),并进行病理组织学检查。结果首乌延寿丹能明显提高缺血脑组织中SOD、CAT活性,降低MDA含量,减轻脑水肿,首乌延寿丹能改善脑缺血造成的大鼠脑组织损伤。结论首乌延寿丹对大鼠局灶性脑缺血有明显的保护作用,其机制可能是通过有效的拮抗自由基损伤,提高脑组织抗氧化能力来实现的。

Protective effects of shouwuy an shoudan on focal cerebral ischemia in rats

Objective To study the protective function of study shouwuyishoudan on focal cerebral ischemia in rats.Methods The middle cerebral artery(MCA)was occluded with thread according to modified of Zealonga's method.The rats were randomly divided into five groups:sham-operated group,ischemia model group,shouwuyishoudan pretreatment group.The contents of MDA,the activities of SOD,CAT in the brain tissue were determined and the pathological section of brain tissue was observed.Results Shouwuyishoudan can significantly improve the activities of SOD and CAT in brain tissue,lower the content of MDA,reduce the brain edema.The observation on the pathological section of brain tissue indicated that shouwuyishoudan could alleviate the injury of cerebral ischemia.Conclusion Shouwuyishoudan has an obvious protective effect on cerebral ischemia in rats,and its treatment mechanism may come from effectively scavenging free radicals and improving the anti-oxidative ability of the brain tissue.