Enhanced SUMOylation and SENP-1 protein levels following oxygen and glucose deprivation in neurones |
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Authors: | Helena Cimarosti Emi Ashikaga Nadia Jaafari Laura Dearden Philip Rubin Kevin A Wilkinson Jeremy M Henley |
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Institution: | 1MRC Centre for Synaptic Plasticity, School of Biochemistry, University of Bristol, University Walk, Bristol, UK |
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Abstract: | Here, we show that oxygen and glucose deprivation (OGD) causes increased small ubiquitin-like modifier (SUMO)-1 and SUMO-2/3 conjugation to substrate proteins in cultured hippocampal neurones. Surprisingly, the SUMO protease SENP-1, which removes SUMO from conjugated proteins, was also increased by OGD, suggesting that the neuronal response to OGD involves a complex interplay between SUMOylation and deSUMOylation. Importantly, decreasing global SUMOylation in cultured hippocampal neurones by overexpression of the catalytic domain of SENP-1 increased neuronal vulnerability to OGD-induced cell death. Taken together, these results suggest a neuroprotective role for neuronal SUMOylation after OGD. |
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Keywords: | ischemia neuronal culture oxygen and glucose deprivation (OGD) posttranslational modification SENP-1 SUMO |
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