| 白细胞介素Ⅰ的检测及白芍总甙对其产生的影响 |
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| 引用本文： | 梁君山,魏伟,周爱武,陈敏珠,徐叔云.白细胞介素Ⅰ的检测及白芍总甙对其产生的影响[J].中国药理学通报,1989(6). |
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| 作者姓名： | 梁君山 魏伟 周爱武 陈敏珠 徐叔云 |
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| 作者单位： | 安徽医科大学临床药理研究所 安徽医科大学临床药理研究所 合肥 |
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| 摘 要： | 本文对小鼠胸腺细胞增殖法检测IL—1的条件及白芍总甙(TGP)对其产生的影响进行了探讨。结果表明,LPS诱导大鼠腹腔Mφ产生IL—1的最佳浓度为8mg/L,诱导出富含IL—1的上清经活化的小鼠脾细胞检测表明无明显IL—2污染。富含IL—1的待测上清协同亚适量Con A诱导小鼠胸腺细胞增殖反应的最佳稀释度为1:90。TGP和亚适浓度LPS与大鼠腹腔Mφ体外共育6h后洗去药物和LPS,结果表明,TGP(0.5～12.5mg/L)可浓度依赖性地增加IL—1的产生,但高浓度TGP(62.5～125mg/L)时,IL—1产生显著降低,量效曲线呈钟罩形趋势,提示TGP对IL—1的产生具有双向作用。
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| 关 键 词： | 白芍总甙 白细胞介素Ⅰ 巨噬细胞 |
METHOD FOR MEASURING IL-1 AND EFFECTS OF TOTAL GLUCOSIDES OF PAEONY ( TGP ) ON IL-1 PRODUCTION |
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| Abstract: | Method for measuring interleukin 1 ( IL -1 ) and effects of total glucosides of paeony(TGP) on IL -1 production were studied. The results showed that the best concentration for LPS -induced IL-1 production of M s in rats was 8mg/L, the best dilution for IL-1 of LPS-induced M s production was 1 : 90. TGP ( 0 .5 - 12 .5mg/L ) enhanced LPS-induced IL -1 production in a concentration dependent manner, but diminished in the higher concentration of TGP ( 62 .5~l25mg/L ) , the concentration-effect curve seems to be bell-shaped. The data suggested that TGP had two-sided regulatory effects on LPS -induced IL production of M s in rats. |
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| Keywords: | Interlereukin l Macrophage Total glucosides of paeony |
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