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| 妊娠早期人外周血及蜕膜局部单核细胞异质性分析 | |
| 引用本文： | 张韵,孙锦堂,黄先亮,杨关香,邵倩倩,邓碧萍,徐小飞,毛海婷,董白桦,孔北华,曲迅.妊娠早期人外周血及蜕膜局部单核细胞异质性分析[J].山东大学学报(医学版),2010,48(3):98-101. |
| 作者姓名： | 张韵 孙锦堂 黄先亮 杨关香 邵倩倩 邓碧萍 徐小飞 毛海婷 董白桦 孔北华 曲迅 |
| 作者单位： | 1. 山东省医学科学院基础医学研究所,济南250062;山东大学齐鲁医院临床基础研究所,济南250012 2. 山东大学齐鲁医院临床基础研究所,济南,250012 3. 山东大学齐鲁医院妇产科,济南,250012 |
| 基金项目： | 国家自然科学基金资助课题(30872321,30671902); 山东省自然科学基金资助课题(Y2008C02) |
| 摘 要： | 目的 分析早孕妇女外周血及蜕膜局部CD14+CD16+/CD14+CD16-单核细胞各亚群的分布，并初步探讨其临床意义。方法 随机抽取健康早孕者及健康未孕者外周血，取人工流产术获得的健康早孕妇女新鲜蜕膜组织，采用机械研磨及密度梯度离心法分离单个核细胞;流式细胞术检测上述样本CD14+CD16+/CD14+CD16-单核细胞各亚群的分布。结果 与健康未孕者比较，早孕妇女外周血CD14+CD16-细胞阳性率显著降低（P＜0.01），早孕蜕膜组织中CD14+CD16+细胞亚群为CD14+细胞的主要群体，阳性率为(69.13±7.73) %，显著高于外周血中该亚群（11.50±3.7）%的比例。结论 早孕期间母体CD14+CD16+细胞亚群成为CD14+细胞中的主群体，提示CD14+CD16+细胞亚群在妊娠期间免疫系统TH-2优势应答的形成中可能起重要作用。 |
| 关 键 词： | 妊娠初期 外周血 蜕膜 单核细胞 |
| 收稿时间： | 2009-11-10 |
Heterogeneity of human peripheral and decidual monocytes of women in early pregnancy |
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| ZHANG Yun,SUN Jin-tang,HUANG Xian-liang,YANG Mei-xiang,SHAO Qian-qian,DENG Bi-ping,XU Xiao-fei,MAO Hai-ting,DONG Bai-hua,KONG Bei-hua,QU Xun.Heterogeneity of human peripheral and decidual monocytes of women in early pregnancy[J].Journal of Shandong University:Health Sciences,2010,48(3):98-101. | |
| Authors: | ZHANG Yun SUN Jin-tang HUANG Xian-liang YANG Mei-xiang SHAO Qian-qian DENG Bi-ping XU Xiao-fei MAO Hai-ting DONG Bai-hua KONG Bei-hua QU Xun |
| Institution: | 1. Institute of Basic Medical Sciences, Shandong Academy of Medical Sciences, Jinan 250062, China; 2. Basic Medical Science Institute, Qilu Hospital of Shandong University, Jinan 250012, China; 3. Department of Gynecology and Obstetrics, Qilu Hospital of Shandong University, Jinan 250012, China |
| Abstract: | Objective To analyze the distribution of human CD14+CD16-and CD14+CD16+ monocyte subsets in peripheral blood and decidua of women in early pregnancy.Methods Peripheral blood samples were collected from early pregnant and nonpregnant females.Mononuclear cells were isolated from the fresh decidua of early pregnant females by mechanical trituration and Ficoll gradient centrifugation.The fluorescent intensity of the cells was detected by FCM.Results The percentage of the peripheral CD14+CD16-monocyte subtype in... |
| Keywords: | First pregnancy trimester Peripheral blood Decidua Monocyte |
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