溶脲脲原体对小鼠巨噬细胞产生TNF－a的影响

溶脲脲原体（ＵｒｅａｐｌａｓｍａＵｒｅａｌｙｔｉｃｕｍ，Ｕ．Ｕ．）是泌尿生殖道常见的病原体，在Ｕ．Ｕ．上行性感染的动物模型中，已证明睾儿发生明显的病理损伤。本文以对数生长期的Ｕ．Ｕ．及热天活Ｕ．Ｕ．加入培养中的小鼠腹腔Ｍφ（ＰＥＣ－Ｍφ）中，并检测Ｍφ产生ＴＮＦ－α的活性。实验证明对数生长期的活Ｕ．Ｕ．及热灭活Ｕ．Ｕ．可使ＰＥＣ—Ｍφ产生ＴＮＦ—ａ的活性明显高于对照组（Ｐ＜０．０５），且与感染剂量相关．提示Ｕ．Ｕ．感染的动物模型中睾丸的病理损伤可能与局部微环境中细胞因子网络改变有关。

The Effect of Ureaplasma Urealyticum on the Productionof TNF - α by Mouse Macrophage

Ureaplasma Urealyticum (U. U. ) is one of the common pathogens in the urogenital tracts. It has been verified that U. U. infection could lead to significant pathologic changes in testis using an animal model of ascending U. U. infection. In this paper,we reported the effect of U. U. on TNF - a production by mouse peritoneal cavity macrophage (PEC - Mφ). It has been shown that both living U. U. at logarithmic growth phase and the heat inacthiated U. U. can stimulate the production of TNF - a by the mouse PEC -Mφ (P<0. 05),being related to the infection dosage. It has .been suggested that the microenvironment changes,one of which is the increase in TNF -a, might be closely related to the cell focus's pathologic changes in testis of U. U. infected animal model.