NOD1在肥胖糖尿病患者脂肪细胞中的表达及对胰岛素抵抗的作用

【目的】探讨肥胖糖尿病（DM）患者脂肪细胞免疫受体核苷酸结合寡聚化结构域蛋白1（nucleotide-binding oligomerization domain-containing protein，NOD1）的表达与胰岛素抵抗的关系。【方法】取10例肥胖 DM患者（DM组）及10例非DM肥胖患者（NDM组）腹部皮下脂肪细胞，采用Real-time PCR方法检测两组脂肪细胞受体 NOD1的表达，加入 NOD1受体激动剂 iE-DAP，用酶联免疫吸附法（ELISA）检测两组细胞炎性细胞因子白细胞介素（IL）-6、IL-8及单核细胞趋化因子1（MCP-1）水平，2-脱氧-3 H-D-葡萄糖摄入法观察基础状态和 iE-DAP刺激状态的葡萄糖摄取率。【结果】DM组脂肪细胞 NOD1 mRNA的表达显著高于 NDM组（P＜0．05）；iE-DAP刺激下DM组 IL-6、IL-8及 MCP-1的分泌水平均显著高于 NDM组（P ＜0．05），DM组胰岛素刺激状态下脂肪细胞葡萄糖摄取率显著低于 NDM组（P＜0．05）。【结论】NOD1介导的炎症反应可能参与肥胖糖尿病胰岛素抵抗的发生。

Expression of NOD1 in Adipocytes of Obese Patients with Diabetes Mellitus and Its Effect on Insulin Resistance

[Obj ective]To explore the expression of immunologic receptor nucleotide-binding oligomerization domain-containing protein 1（NOD1）in adipocytes of obese patients with diabetes mellitus（DM）and its relation with insulin re-sistance（IR）.[Methods]Abdominal subcutaneous adipocytes were drawn from 10 obese patients with DM（DM group） and 1 0 obese patients without DM（NDM group）.Real-time PCR was used to detect the expression of NOD1 in adipocytes of two groups.NOD1 receptor agonist iE-DAP was administered.Enzyme linked immunosorbent assay（ELISA）was used to detect the levels of inflammatory cytokines such as interleukin-6（IL-6）,interleukin-8（IL-8）and monocyte chemotactic factor-1（MCP-1）in two groups.The baseline state and iE-DAP-stimulated glucose uptake were observed by 2-deoxy-D-3 H glucose uptake assay.[Results]NOD1 mRNA expression in adipocytes of DM group was markedly higher than that of NDM group（P 〈0.05）.The levels pf IL-6,IL-8 and MCP-1 in DM group with iE-DAP stimulation were markedly higher than those in NDM group（P〈0.05）.Adipocyte glucose transport rate in DM group with iE-DAP stim-ulation was significantly lower than that in NDM group（P〈0.05）.[Conclusion]NOD1-mediated inflammatory response may involve in the occurrence of IR in obese DM.