Priming of cytokine release and increased levels of bactericidal permeability-increasing protein in the blood of animal facility workers |
| |
Authors: | P. J. A. Borm B. van Hartingsveld P. F. R. Schins E. Alfaro-Moreno G. Cruz-Rico I. Rosas-Perez A. R. Osornio-Vargas |
| |
Affiliation: | (1) Medical Institute of Environmental Hygiene at the University of Düsseldorf, Department of Particle & Fiber Toxicology, P.O. Box 103751, D-40028 Düsseldorf, Germany Tel.: +49-211 3389 338; Fax: +49-211 3389 331 e-mail: Paul.Borm@uni-duesseldorf.de, DE;(2) Department of Basic Investigation, National Institute of Cancer Study, Mexico, MX;(3) Center of Atmospheric Sciences, National Autonomic University of Mexico City, MX |
| |
Abstract: | Objective: To investigate cellular immune responses in laboratory animal workers who are exposed to high levels of animal allergens but also to other biologically active substances containing lipopolysaccharides (LPS), i.e., endotoxins. Methods: A survey among 20 animal facility workers and 20 matched (gender, smoking) controls was conducted using exposure measurements (endotoxin, colony-forming units of bacteria and fungi) and a questionnaire on respiratory symptoms. Blood samples were taken to determine the ex vivo whole-blood release of tumor necrosis factor-alpha (TNF) and interleukin-8 (IL-8) as well as plasma levels of LPS-binding protein (LBP), bactericidal permeability-increasing protein (BPI), the 75-kDa soluble TNF receptor (sTNFR75), and total/specific IgE. Results: Although exposure to endotoxin was low (range 0.05–2.8 ng/m3), a significant (P < 0.05) increase in plasma BPI (4-fold) and srTNF75 (1.2-fold) was found, suggesting a response to inhalation of LPS. Also, the capacity of blood leukocytes to release TNF and IL-8 in response to ex vivo exposure to workplace dust was increased. Data were not confounded by specific allergies, levels of IgE, smoking, or respiratory symptoms. Conclusions: A profound effect on the cellular immune response was seen in animal workers with low endotoxin exposure and a high antigenic load. It remains to be determined which other biologically active substance(s) are involved in this effect. Received: 4 November 1998 / Accepted: 2 March 1999 |
| |
Keywords: | Animal workers Allergy Cytokines LPS-binding protein BPI IgE Endotoxin |
本文献已被 SpringerLink 等数据库收录! |
|