耐甲氧西林金黄色葡萄球菌的基因分型研究

目的明确本地区耐甲氧西林金黄色葡萄球菌(MRSA)的基因型别及其分布规律,探讨MR-SA菌株进化及遗传特性。方法收集2009年1月—2012年1月鄂尔多斯中心医院门诊及住院患者非痰标本分离的MRSA菌株61株,应用SCCmec基因分型和葡萄球菌A蛋白基因多态性分析(spa)方法进行基因分型。结果应用SCCmec的方法分离的MRSA以SCCmecⅡ、Ⅲ型为主,分别占50.8%和44.3%;应用spa的方法分离的MRSA以t030和t002为主,分别占39.3%和34.4%。结论本地区分离MRSA以SCCmecⅢ-t030和SCCmecⅡ-t002为主。

STUDY ON GENOTYPE OF METHICILLIN - RESISTANT STAPHYLOCOCCUS AUREUS

Objective To track the genetic changes,spread and evolution of MRSA in this region,understand of genotypic characteristics and evolutionary pathway of endemic MRSA isolates.Methods A total of 61 MRSA isolates were recovered from patients presenting with symptoms consistent with MRSA infection by the clinical microbiology laboratory in Ordos center hospital between Jan.2009 and Jan.2012.Genotyping and A gene polymorphism analysis(spa) were analyzed by SCCmec.Results MRSA isolated were SCCmec Ⅱ,Ⅲ type mainly by SCCmec typing,accounted for 50.8% and 44.3% each;MRSA isolated were SCCmec Ⅱ,Ⅲ type mainly by spa typing,accounted for 39.3% and 34.4% each.Conclusion MRSA isolates are mainly SCCmec Ⅲ-t030 and SCCmecⅡ-t002 in Ordos.