HPLC同时测定妇炎康复片3种主要抗炎成分含量

目的:建立HPLC同时测定妇炎康复片中芍药苷、橙皮苷及黄芩苷含量的方法。方法:采用HPLC条件为:色谱柱为Phenomenex C18柱(250mm×4.60mm,5μm);流动相:乙腈-磷酸二氢钾水溶液(0.05mol/L,磷酸调pH至3.0)(梯度洗脱:0min,17∶83;10min,17∶83;15min,22∶78;30min,30∶70);流速:1.0mL/min;柱温:40℃;检测波长:231nm。结果:芍药苷的线性范围为0.0533～0.4800μg(r=0.9997),橙皮苷的线性范围为0.0798～0.7182μg(r=0.9999),黄芩苷的线性范围为0.3600～3.420μg(r=0.9999);平均加样回收率,芍药苷为99.49%,RSD=1.70%(n=5),橙皮苷为99.59%,RSD=0.41%(n=5),黄芩苷为99.92%,RSD=1.26%(n=5)。结果测得3厂家妇炎康复片中每片含芍药苷为1.0656,1.4455,0.7042mg;橙皮苷为0.5383,0.7676,0.4403mg;黄芩苷为3.9544,7.0312,2.8877mg。结论:该方法简便、快速、准确,可用于妇炎康复片的质量控制。

Simultaneous Determination of Three Main Anti-inflammatory Constituents in Fuyankangfu Tablets by HPLC

Objective To establish a HPLC method for determining the contents of paeoniflorin, hesperidin and baicalin in Fuyankangfu Tablets. Methods HPLC was performed on a Phenomenex-C18 column（ 250 mm× 4.60 mm,5 um） , and the mobile phase was composed of aeetonitrile4）. 05mol/L KH2PO4 （adjusted to pH 3.00 with H3PO4 ）with gradient elution （0 min, 17：83 ; 10 min, 17：83 ; 15 rain,22：78 ;30min,30： 70）. The flow-rate was 1.0 mL/min. Tile operating temperatore was maintained at 40℃ and the detection wavelength was 231 nm. Results The linear ranges of paeoniflorin, hesperidin and baicalin were 0. 0533 - 0. 4800 ug（ r = 0. 9997 ）, 0.0798 - 0. 7182 ug（ r = 0. 9999 ） and 0.3600 - 3. 4200 ug （ r = 0. 9999）, respectively. The average recovery rate of paeoniflorin was 99.49% （ RSD = 1.70%, n = 5 ）, hesperidin was 99.59% （ RSD = 0.41%, n = 5 ） and baicalin was 99.92% （ RSD = 1.26%, n = 5 ）. The contents of paeoniflorin in Fuyankangfu Tablets from three companies were.,1. 0656,1. 4455,0. 7042 rag, hesperidin were 0. 5383,0. 7676,0. 4403 mg, baiealin were 3. 9544,7.0312,2. 8877 nag, respectively. Conclusion The method is simple, quick, accurate, which can be used for the quantity control of Fuyankangfu Tablets.