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| 大鼠海马神经干细胞的分离培养与免疫荧光鉴定 | |
| 引用本文： | 陈凯,康现江,张平,穆淑梅,郭明申. 大鼠海马神经干细胞的分离培养与免疫荧光鉴定[J]. 河北职工医学院学报, 2010, 27(2): 1-3,16 |
| 作者姓名： | 陈凯 康现江 张平 穆淑梅 郭明申 |
| 作者单位： | 1. 河北大学,生命科学学院,河北,保定,071002;河北工程大学,农学院,河北,邯郸,056001 2. 河北大学,生命科学学院,河北,保定,071002 3. 河北大学附属医院,神经内科,河北,保定,071000 |
| 基金项目： | 河北省科技厅基金项目 |
| 摘 要： | 目的分离培养新生SD大鼠神经干细胞,并对其生物学特性进行鉴定。方法采用无血清培养联合应用碱性成纤维细胞生长因子和表皮生长因子对大鼠海马神经干细胞分离培养,采用免疫荧光的方法检测神经干细胞的nestin抗原。结果从新生大鼠海马齿状回分离的细胞群可不断增殖形成细胞克隆球,并且呈nestin阳性表达,具有多向分化能力。结论分离培养的细胞是中枢神经系统的干细胞。 |
| 关 键 词： | 神经干细胞 分离培养 免疫荧光鉴定 |
Isolation,culture and immunofluorescence identification of neural stem cells in rats |
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| Chen Kai,,Kang Xianjiang,Zhang Ping,et al.. Isolation,culture and immunofluorescence identification of neural stem cells in rats[J]. Journal of Hebei Medical College for Continuing Education, 2010, 27(2): 1-3,16 | |
| Authors: | Chen Kai Kang Xianjiang Zhang Ping et al. |
| Affiliation: | Chen Kai1,2,Kang Xianjiang1,Zhang Ping3,et al. (1. College of Life-sciences,Hebei University,Baoding Hebei 071002,2. College of Agriculture,Hebei University of Engineering,H,an Hebei 056001,3. Department of Neurology,Affiliated Hospital of Hebei University,Baoding Hebei 071000,China) |
| Abstract: | Objective To isolate and culture neural stem cells from SD newborn rats,and identify its biological properties. Methods This study adopted the serum -free culture combined with the basic fibroblast growth factor and epidermal growth factor to isolate and culture the rat hippocampal neural stem cells, using immunofluorescence method to detect the nestin antigen of neural stem cells. Results The results showed that the cells isolated from the hippocampal dentate gyrus could proliferate continuously,and form t... |
| Keywords: | neural stem cell isolation and culture immunofluorescence |
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