更昔洛韦和黄芪对巨细胞病毒感染所致造血祖细胞增殖抑制的影响

目的　探讨更昔洛韦 (GCV)和黄芪对巨细胞病毒 (CMV)感染所致脐血粒 巨噬系祖细胞 (CFU GM)、红系早、晚期祖细胞 (CFU E、BFU E)、多向造血祖细胞 (CFU Mix)及巨核系祖细胞(CFU Mk)体外增殖抑制的影响。方法　采用造血祖细胞培养技术 ,培养、观察、计数巨细胞病毒AD169株 (CMV AD169)感染CFU GM、CFU E、BFU E、CFU Mix和CFU Mk后各祖细胞集落、峰期及维持时间 ;用聚合酶链反应 (PCR)检测集落细胞内CMV DNA ;根据细胞毒性实验结果 ,将GCV和黄芪作用于CMV感染的CFU GM、CFU E、BFU E、CFU Mix及CFU Mk祖细胞 ,再分别计数集落、峰期及维持时间。结果 ( 1)CMV感染组CFU GM、CFU E、BFU E、CFU Mix及CFU Mk集落数较对照组明显减少 (P <0 0 1) ;集落维持时间CMV感染组较对照组明显缩短 ;( 2 )用PCR在感染组集落细胞内检测到CMV DNA的存在 ;( 3)黄芪和 (或 )GCV作用于CMV感染的祖细胞后 ,与病毒组比较集落维持时间明显延长 ,集落数和集落增殖率明显提高 (P <0 0 1) ,黄芪组集落增殖率分别为 2 7 2 %、4 5 2 %、4 9 1%、39 0 %、11 9% ;GCV组分别为 37 4 %、74 2 %、71 7%、6 7 4 %、38 9% ;GCV 黄芪组分别为 5 3 6 %、83 8%、88 7%、87 8%、6 1 5 %。结论　CMV AD169在体外能明显抑制CFU GM、CFU E、BFU

Influence of ganciclovir and astragalus membranaceus on proliferation of hematopoietic progenitor cells of cord blood after cytomegalovirus infection in vitro

Objective Cytomegalovirus(CMV)infection was greatly common in the world. CMV infection produces usually mild or asymptomatic infections in individuals with normal immune responses, whereas it may cause serious disease in immunosuppressive patients. Clinical manifestations include suppression of myelopoiesis, a mononucleosis like syndrome, hepatosplenomegaly, lymphadenopathy, thrombocytopenia, and hemolytic anemia. In patients undergoing bone marrow transplantation CMV remains the most common infectious causes of morbidity and mortality. But the treatment drugs with specific effect for CMV was fewer at the present. This study was to investigate the effect of CMV on proliferation of colony forming unit granulocyte macrophage(CFU GM), CFU erythroid(CFU E), brust forming unit erythroid (BFU E),CFU multipotential(CFU Mix) and CFU megakaryocyte (CFU Mk) progenitor cells of cord blood (CB) with the presence of ganciclovir (GCV) and astragalus membranaceus in vitro.Methods Twenty CB samples were collected from fetal umbilical vein of normal term spontaneous delivery neonates. The colony forming unit assay was applied to observe the suppression effect of CMV AD 169 strain on CFU GM, CFU E, BFU E, CFU Mix and CFU Mk of CB with the presence of GCV and astragalus membranaceus in vitro. The technique of PCR was used to demonstrate the existence of CMV AD 169 DNA in the colony cells of cultured CFU GM, CFU E, BFU E, CFU Mix and CFU Mk. Results (1) The numbers of CFU GM, CFU E, BFU E, CFU Mix and CFU Mk colonies in CMV infection groups were significantly less than those in blank and mock group, respectively. The last time of colonies in groups with CMV infection was significantly shorten compared with the blank and mock group. (2) CMV DNA was positively detected in the colony cells of CMV infection groups by PCR, while negative in the control groups.(3) The lasting time of CFU GM, CFU E, BFU E, CFU Mix and CFU Mk colonies infected with CMV extended significantly with the presence of astragalus membranaceus and GCV, and the numbers of those increased significantly compared with the CMV infection group, respectively. The increasing rate of colonies was 27.2%, 45 2%, 49 1%, 39 0% and 11 9% with astragalus membranaceus group, 37 4%, 74 2%, 71 7%, 67 4% and 38 9% with GCV group, 53 6%, 83 8%, 88 7%, 87 8% and 61 5% with astragalus membranaceus and GCV group, respectively. Conclusions The differentiation and proliferation of CFU GM, CFU E, BFU E, CFU Mix and CFU Mk were significantly inhibited after infected with CMV AD 169 strain. The suppression effect of CMV AD 169 on CFU GM, CFU E, BFU E, CFU Mix and CFU Mk was inhibited with the presence of GCV and astragalus membranaceus in vitro. This suggested that CMV AD 169 may be inhibited or killed by GCV and Astragalus Membranaceus in vitro.