胃癌及癌前病变中肝肠钙黏蛋白的表达及其意义

目的 研究胃癌及肠化生组织中肝肠钙黏蛋白(liver-intestine cadherin. LI-cadherin)的表达,探讨胃黏膜癌前病变及癌变的分子机制. 方法应用SP免疫组化方法检测244例不同胃组织中(包括正常胃黏膜28例、慢性浅表性胃炎30例、慢性萎缩性胃炎42例、肠化生胃黏膜58例、胃腺癌46例、癌旁组织30例、胃间质瘤10例) LI-cadherin蛋白的表达.结果 LI-cadherin蛋白在正常胃黏膜、慢性胃炎、癌旁组织及胃间质瘤组织中不表达;在肠化生组织中阳性表达率为83%,在胃腺癌组织中阳性表达率为65%.按Laurien分型,肠型胃癌中LI-cadherin蛋白阳性表达率(78%)高于弥漫型胃癌(35%),两者之间差异有统计学意义(P<0.05).LI-cadherin与淋巴结转移及临床分期明显正相关(P<0.01). 结论LI-cadherin的异常表达与肠化生及胃腺癌的发生有关;LI-cadherin高表达者淋巴结转移率高.有助于判断胃癌恶性程度及预后.

A study on the expression of LI-cadherin in precancerous lesion and gastric cancer

Objective To study the expression of LI-cadherin in gastric cancer, gastric stromal tumor, chronic gastritis and intestinal metaplasia. Methods Two hundred and forty four specimens were collected, including normal epithelia (n=28), chronic superficial gastritis (n=30), chronic atrophic gastritis(n=42), intestinal metaplasia (n=58), gastric adenocarcinoma (n=46), paracancerous gastric tissues (n=30), gastric stromal tumor (n=10). The expression of LI-cadherin was detected by S-P immunohischemistry with purified goat polyclonal antibody. Results The expression of LI-cadherin in normal epithelia and chronic gastritis are all negative, the positive rates of LI-cadherin expression in intestinal metaplasia and gastric adenocarcinoma is 83% (48/58) and 65% (30/46) respectively. By Laurien classification, the positive rate of LI-cadherin expression in intestinal type was higher(78% ) than those in the diffuse type (35%) (P<0.05). LI-cadherin was in positive correlation with lymph node metastasis and staging. Paracancerous tissues and gastric stromal tumor did not express LI-cadherin. Conclusions The abnormal expression of LI-cadherin was correlated with intestinal metaplasia and gastric adenocarcinoma. GCs with high LI-cadherin index have more lymph node metastasis. High expression rate of LI-cadherin in gastric cancer tissues may predict poor prognosis.